Anti-IST1 Polyclonal Antibody for WB, ELISA - P53990

Core Product Specifications and Parameters

Biological Background: IST1 Function and Localization

• IST1 homolog (also known as CHMP8/OLC1) is an ESCRT-III-like protein crucial for membrane remodeling processes including cytokinesis, nuclear envelope reassembly, and endosomal tubulation.
  Related references:
  PMID:19129479
  PMID:26040712
  PMID:28242692

• During cytokinesis, IST1 is required for efficient abscission and recruits VPS4A/VPS4B to the midbody to drive the final separation of daughter cells.
  Related references:
  PMID:19129479
  PMID:19129480

• In late anaphase, IST1 mediates nuclear envelope reassembly and mitotic spindle disassembly by targeting the microtubule-severing enzyme SPAST to the reforming nuclear membrane.
  Related references:
  PMID:26040712

• IST1 is actively recruited to the nuclear envelope during anaphase by the inner nuclear membrane protein LEMD2, facilitating its role in nuclear reformation.
  Related references:
  PMID:28242692

• The protein also regulates early endosomal tubulation in coordination with the ESCRT-III complex by recruiting SPAST, linking ESCRT machinery to endosomal dynamics.
  Related references:
  PMID:23897888

• Subcellularly, IST1 localizes to cytoplasmic vesicles, the centrosome, the midbody, and the nuclear envelope, consistent with its diverse roles in membrane fission and remodeling.

• IST1 is subject to alternative splicing and phosphorylation, and is classified under cell cycle, cell division, and nuclear dynamics keywords.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-335 of human IST1 (NP_001257906.1), suggesting the antibody can detect full-length IST1 (predicted ~40 kDa) in human, mouse, and rat lysates.
• For Western blot, validate the antibody in relevant cell lines or tissues and optimize using a positive control (e.g., HeLa or HEK293 cells). Non-specific bands may be minimized by adjusting blocking and washing conditions.
• For ELISA, test the antibody as a capture or detection reagent with appropriate antigen coating concentrations and detection systems.
• Ensure proper sample handling and lysis conditions to preserve IST1 protein integrity and localization-dependent interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research institutions with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents for immunoassay development. Our expertise extends to rare target sourcing and technical consultation, streamlining your path from concept to clinical application. This anti-IST1 polyclonal antibody exemplifies our commitment to supplying high-quality tools for studying ESCRT-III-mediated processes and cell division signaling.