Anti-Isovaleryl-CoA Dehydrogenase (IVD) Rabbit Polyclonal Antibody for WB, ELISA - P26440

Core Product Specifications and Parameters

Biological Background: Isovaleryl-CoA Dehydrogenase (IVD) Function and Localization

• Isovaleryl-CoA dehydrogenase (IVD), also known as butyryl-CoA dehydrogenase, is a mitochondrial matrix enzyme encoded by the IVD gene. It belongs to the acyl-CoA dehydrogenase family and plays dual roles in amino acid and lipid metabolism.
• IVD catalyzes the third step in leucine catabolism by converting isovaleryl-CoA (3-methylbutanoyl-CoA) to 3-methylbut-2-enoyl-CoA. It also participates in the first step of fatty acid beta-oxidation, catalyzing the proR-proR stereospecific alpha,beta-dehydrogenation of short-chain acyl-CoA thioesters (e.g., pentanoyl-CoA, hexanoyl-CoA, butanoyl-CoA) using electron transfer flavoprotein (ETF) as the physiological electron acceptor.
  Related references: PMID:7640268
• IVD is localized exclusively in the mitochondrial matrix, where it functions as a key metabolic enzyme.
• As a flavoprotein, IVD requires FAD as a cofactor for its dehydrogenase activity.
• UniProtKB annotates disease-associated variants in IVD, linking it to metabolic disorders such as isovaleric acidemia. However, specific clinical correlations require further validation.
• The protein undergoes post-translational modifications including acetylation and is subject to alternative splicing.
• The full-length precursor has a calculated molecular mass of 46.7 kDa, consistent with the ~47 kDa mature form observed experimentally.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody covers amino acids 33–160 of human IVD, corresponding to a region that may include both structured and surface-exposed domains. This design may enable recognition of both native and denatured protein conformations in applications like WB and ELISA.
• For Western blotting, a band around 47 kDa is expected for human IVD. Consider optimizing loading controls and lysis conditions, especially when working with mitochondrial fractions.
• When performing ELISA, validate the antibody against recombinant IVD protein or cell lysates from species of interest, noting the reported cross-reactivity with mouse and rat.
• Since cross-reactivity is indicated for mouse and rat, this antibody may be suitable for comparative studies across species; however, individual optimization is recommended.
• All shipping and storage conditions assume cold chain (ice bag); aliquot and store at -20°C or lower upon arrival to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials and technical services, streamlining the transition from concept to clinical application. For studies on mitochondrial metabolism, fatty acid oxidation, and inherited metabolic diseases like isovaleric acidemia, we offer validated antibody pairs, optimized polyclonal antibodies such as this anti-IVD antibody, and bulk ancillary reagents. Our sourcing capabilities extend to rare and emerging targets, ensuring supply chain continuity for your assay development needs.