Anti-Islet1 Rabbit Polyclonal Antibody for WB, ELISA - P61371

Core Product Specifications and Parameters

Biological Background: Islet1 Function and Localization

• Islet1 (Insulin gene enhancer protein ISL-1) is a DNA-binding transcriptional activator that recognizes the consensus octamer motif 5'-ATAATTAA-3' in target gene promoters.
• It plays a fundamental role in retinal ganglion cell (RGC) differentiation, cooperating with POU4F2 to drive RGC fate specification in the developing retina (By similarity).
• Islet1 is essential for heart development; it marks a progenitor population contributing to the outflow tract, right ventricle, and atrial cells, and is required for cardiac progenitor migration and proliferation (By similarity).
• In motor neuron specification, Islet1 cooperates with LHX3 and LDB1 to promote motor neuron identity (By similarity).
• The protein localizes to the nucleus, consistent with its function as a transcription factor.
• Islet1 contains LIM domains, a homeobox DNA-binding domain, and is subject to post-translational modifications including phosphorylation and ubiquitination.
• It is expressed in subsets of neurons, including adrenal medulla, dorsal root ganglion, retina, pineal gland, and certain brain regions, highlighting its role in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 1-349 of human Islet1; consider this when designing epitope-specific assays.
• For Western blot, a band near 39 kDa is expected; validate detection in your specific sample lysates (e.g., human, mouse, rat tissues or cell lines expressing Islet1).
• In ELISA applications, the antibody can be used for antigen capture or detection; optimize coating and detection conditions for your assay format.
• Cross-reactivity is reported for human, mouse, and rat; sequence homology suggests potential reactivity with other species, but experimental validation is recommended.
• Islet1 expression is tissue- and stage-specific; choose appropriate positive controls (e.g., developing retina, heart, or motor neuron cultures).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering high-quality antibodies like this anti-Islet1 polyclonal antibody to support diagnostic development and life science research. With expertise in transcription factor targets and developmental biology reagents, we supply validated antibody pairs, recombinant proteins, and ancillary reagents in bulk. Our team can also assist in sourcing rare raw materials for Islet1 and related targets, streamlining your path from concept to clinic.