Anti-ISG20 Rabbit Polyclonal Antibody for WB, ELISA - Q96AZ6

Core Product Specifications and Parameters

Biological Background: ISG20 Function and Localization

• ISG20 (Interferon-stimulated gene 20 kDa protein), also known as HEM45 or CD25, is an interferon-induced antiviral exoribonuclease that primarily acts on single-stranded RNA. Related references: PMID:11401564 PMID:12594219 PMID:16033969
• It exhibits antiviral activity against RNA viruses including hepatitis C virus (HCV), hepatitis A virus (HAV), and yellow fever virus (YFV). For certain viruses like chikungunya virus, inhibition involves translational suppression of viral proteins rather than RNA degradation. Related references: PMID:16514659 PMID:21036379
• ISG20 localizes to multiple subcellular compartments: nucleus, nucleolus, Cajal bodies, cytoplasm, and processing bodies (P-bodies), where it exerts translational control over non-self RNA substrates while sparing endogenous transcripts. Related references: PMID:31600344
• The protein may function as a master regulator of over a hundred interferon-stimulated genes, leading to broad inhibition of viral genome translation.
• ISG20 may also participate in snRNA and rRNA maturation and ribosome biogenesis. Related references: PMID:16514659
• It contains an exonuclease domain and requires manganese as a cofactor (Keywords: Manganese, Metal-binding, Exonuclease, Hydrolase).
• ISG20 is highly expressed in peripheral blood leukocytes, spleen, thymus, colon, and lung, and is upregulated by estrogen in estrogen receptor-positive breast cancer lines.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of ISG20 is 20 kDa. Consider using a 15% SDS-PAGE gel for optimal resolution of low-molecular-weight proteins.
• In ELISA assays, the recombinant protein immunogen (amino acids 1-70) can be used as a positive control to establish standard curves.
• When analyzing mouse or rat samples, validate the antibody's specificity in the relevant species, as sequence homology may lead to reactivity, but expression levels or post-translational modifications could differ.
• Given that ISG20 expression is induced by interferon, consider treating cells with type I interferon to upregulate target expression before detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-ISG20 antibody is suitable for research and assay development in antiviral innate immunity and interferon signaling. For your project needs, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support sourcing of rare targets.