Anti-IRS2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y4H2

Core Product Specifications and Parameters

Biological Background: IRS2 Function and Localization

• IRS2 (Insulin receptor substrate 2) is a signaling adapter protein that mediates signal transduction from insulin receptor (INSR) and insulin-like growth factor I receptor (IGF1R), playing critical roles in development, growth, glucose homeostasis, and lipid metabolism. Related references: PMID:25879670, PMID:24616100
• Upon phosphorylation by the insulin receptor, IRS2 serves as a docking scaffold for SH2 domain-containing proteins, including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2, and SHP2, which are essential for downstream signaling. Related references: PMID:15316008, PMID:19109239
• IRS2 activates the PI3K/AKT pathway, which mediates most of the metabolic effects of insulin, and the Ras/Raf/MEK/MAPK cascade, which regulates gene expression and, in cooperation with PI3K, cell growth and differentiation.
• IRS2 positively regulates the Wnt/β-catenin signaling pathway by suppressing DVL2 autophagy-mediated degradation, leading to enhanced cell proliferation. Related references: PMID:24616100
• IRS2 contributes to cell cycle progression by promoting a robust spindle assembly checkpoint (SAC) during M-phase. Related references: PMID:32554797
• In macrophages, interleukin-4 (IL4)-induced tyrosine phosphorylation of IRS2 leads to recruitment and activation of phosphoinositide 3-kinase (PI3K), highlighting its role in immune signaling. Related references: PMID:19109239
• IRS2 is predominantly localized in the cytoplasm and cytosol.
• Post-translational modifications of IRS2 include phosphorylation, methylation, isopeptide bond formation, and ubiquitin-like conjugation, which modulate its activity and stability. Keywords: Phosphoprotein, Methylation, Isopeptide bond, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1238–1338 near the C-terminus of human IRS2. This region may be well conserved across species, consistent with the observed cross-reactivity with mouse and rat.
• For Western blot (WB), IRS2 has a predicted molecular weight of 137 kDa. Use a low-percentage polyacrylamide gel (e.g., 6–8%) and extended transfer times to ensure efficient transfer of high-molecular-weight proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), verify antibody specificity using appropriate controls, as polyclonal antibodies may exhibit nonspecific binding.
• For ELISA, optimize coating and detection conditions with recombinant IRS2 protein or cell lysates to achieve the best signal-to-noise ratio.
• As a polyclonal antibody produced in rabbit, it may detect multiple isoforms or degradation products; confirm the expected band pattern in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutions from concept to clinic. IRS2, as a key mediator of insulin signaling and metabolic pathways, is widely studied in diabetes, obesity, and cancer research. CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare-target raw materials to streamline your assay development.