Anti-IRS2 Monoclonal Antibody for WB, IF/ICC, ELISA - Q9Y4H2

Core Product Specifications and Parameters

Biological Background: IRS2 Function and Localization

• IRS2 (Insulin receptor substrate 2) is a signaling adapter protein that mediates signal transduction from insulin receptor (INSR) and insulin-like growth factor I receptor (IGF1R), playing a critical role in development, growth, glucose homeostasis, and lipid metabolism. Related references: PMID:24616100
• Upon phosphorylation by the insulin receptor, IRS2 functions as a scaffold that recruits SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, leading to activation of the PI3K/AKT pathway, which is responsible for most insulin metabolic effects. Related references: PMID:25879670
• Recruitment of GRB2 by IRS2 triggers the Ras/Raf/MEK/MAPK signaling cascade, involved in the regulation of gene expression, cell growth, and differentiation.
• IRS2 also acts as a positive regulator of the Wnt/β-catenin signaling pathway by suppressing DVL2 autophagy-mediated degradation, thereby promoting cell proliferation. Related references: PMID:24616100
• During M-phase, IRS2 contributes to cell cycle progression by promoting a robust spindle assembly checkpoint (SAC). Related references: PMID:32554797
• In macrophages, IL4-induced tyrosine phosphorylation of IRS2 leads to the recruitment and activation of phosphoinositide 3-kinase (PI3K), linking it to immune signaling. Related references: PMID:19109239
• Localized predominantly in the cytoplasm (cytosol), IRS2 undergoes post-translational modifications including phosphorylation, methylation, and ubiquitination, reflecting its dynamic regulation.
• As a reference proteome protein with a molecular weight of approximately 137 kDa, IRS2 is a key transducer in insulin and growth factor signaling.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from human IRS2 C-terminus (aa 1239-1338). For Western blot, consider testing the antibody against whole-cell lysates from IRS2-expressing cell lines (e.g., HepG2, HEK293) and validate with a suitable loading control. The predicted molecular weight is ~137 kDa; ensure appropriate gel percentage for resolution.
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions as IRS2 is cytosolic. Use a permeabilization step (e.g., 0.1% Triton X-100) and include appropriate negative controls.
• In ELISA applications, pairing with a validated capture/detection antibody may enhance sensitivity; consider checkerboard titrations to determine optimal antibody concentrations and coating conditions.
• Cross-reactivity with mouse and rat IRS2 is indicated; confirm reactivity in your specific model system using tissue or cell extracts before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing solutions. For researchers and diagnostic developers focusing on insulin signaling, metabolic disorders, and growth factor pathways, CamelBio facilitates rapid access to high-quality IRS2-related reagents and technical support, accelerating your project from concept to clinic.