Anti-IRS1 Rabbit Polyclonal Antibody for WB and ELISA - P35569

Core Product Specifications and Parameters

Biological Background: IRS1 Function and Localization

• Insulin receptor substrate 1 (IRS1) is a signaling adapter protein that mediates signal transduction from the insulin receptor (INSR) and insulin-like growth factor I receptor (IGF1R), playing a pivotal role in development, growth, glucose homeostasis, and lipid metabolism. Related references: PMID:7526222
• Upon phosphorylation by activated insulin receptor, IRS1 serves as a scaffold for SH2 domain-containing proteins such as the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2, or SHP2, initiating diverse downstream cascades.
• Recruitment of GRB2 activates the Ras/Raf/MEK/MAPK signaling cascade, which regulates gene expression and cooperates with the PI3K pathway to control cell growth and differentiation.
• Activation of the PI3K/AKT pathway is responsible for most metabolic effects of insulin, including glucose uptake and glycogen synthesis.
• IRS1 positively regulates the Wnt/beta-catenin signaling pathway by suppressing autophagy-mediated degradation of DVL2, thereby promoting cell proliferation.
• Subcellularly, IRS1 localizes to both the cytoplasm and nucleus, consistent with its role in signal relay and transcriptional regulation.
• Tissue-specific expression has been documented in osteoblasts but not in osteoclasts, suggesting a role in bone metabolism.
• Key post-translational modifications include phosphorylation on multiple tyrosine and serine/threonine residues, as well as ubiquitination and S-nitrosylation, which modulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 1134-1233 of mouse IRS1; therefore, consider verifying detection of full-length and potential truncated isoforms in your sample system.
• For Western blotting, note the high predicted molecular weight (~131 kDa); standard transfer conditions may need optimization, and gradient gels could improve resolution.
• Validate the antibody’s reactivity in your specific mouse tissue or cell lysates, as cross-reactivity with other species is not indicated.
• In ELISA applications, ensure the coating antigen or capture format is compatible with the polyclonal nature of the antibody, and consider blocking to minimize nonspecific binding.
• As this is a polyclonal antibody, lot-to-lot variability should be assessed when establishing long-term assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. For those exploring insulin signaling, glucose metabolism, or related pathways, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Our support framework is designed to streamline your procurement process, ensuring you have reliable materials for assay development and production.