Anti-IRP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P48200

Core Product Specifications and Parameters

Biological Background: IRP2 Function and Localization

• Encodes iron-responsive element-binding protein 2 (IRP2), also known as iron regulatory protein 2; alternative splicing produces multiple isoforms.
• Binds to iron-responsive elements (IREs) in the 5'-UTR of ferritin and delta-aminolevulinic acid synthase mRNAs, repressing translation under low iron conditions.
• Binds to IREs in the 3'-UTR of transferrin receptor mRNA, stabilizing the transcript and increasing receptor expression.
• Contains a 4Fe-4S iron-sulfur cluster that acts as an iron sensor, modulating RNA-binding activity.
• Localizes predominantly to the cytoplasm, consistent with its role in post-transcriptional regulation.
• Genetic variants in IREB2 (the encoding gene) are linked to neurodegenerative disorders, as indicated by the disease variant keyword.
• Undergoes ubiquitination (Ubl conjugation) and other post-translational modifications that likely regulate its stability and activity.

Experimental Guidance and Technical Tips

• The immunogen spans IRP2 residues 760–963; the antibody is expected to recognize both full-length IRP2 (~105 kDa) and potential isoforms in human, mouse, and rat samples.
• For Western blotting, consider using lysates from cells treated with iron chelators (e.g., DFO) to stabilize IRP2 versus iron-replete conditions to confirm band specificity.
• In immunofluorescence, a predominantly cytoplasmic signal is anticipated; co-staining with a nuclear marker (e.g., DAPI) and a known cytoplasmic marker can help validate localization.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; perform titration for each new lot and application (WB, IF/ICC, or ELISA).
• Cross-reactivity with mouse and rat has been reported, but sequence alignment around the immunogen region is recommended to predict reactivity in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This IRP2 polyclonal antibody exemplifies our commitment to providing high-quality immunological tools for iron metabolism and neurodegenerative disease research. Whether you require monoclonal alternatives, conjugated formats, or custom rare-target sourcing, CamelBio bridges your concept-to-clinic needs.