Anti-IRP2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P48200

Core Product Specifications and Parameters

Biological Background: IRP2 Function and Localization

• IRP2 (iron-responsive element-binding protein 2), also known as iron regulatory protein 2, is encoded by the IREB2 gene. It plays a critical role in cellular iron homeostasis.
• It binds specifically to iron-responsive elements (IREs) in the untranslated regions (UTRs) of target mRNAs, such as ferritin and transferrin receptor, to post-transcriptionally regulate iron uptake, storage, and utilization.
• This RNA-binding protein represses the translation of ferritin mRNA when iron levels are low, while simultaneously stabilizing transferrin receptor mRNA to increase iron import.
• IRP2 contains a 4Fe-4S iron-sulfur cluster and belongs to the aconitase family, consistent with its metal-binding and catalytic properties.
• The protein predominantly localizes to the cytoplasm, where it senses intracellular iron levels and modulates gene expression accordingly.
• Alternative splicing generates multiple isoforms, and post-translational modifications, including ubiquitination (Ubl conjugation), regulate its stability and activity.
• Mutations in IRP2 have been associated with neurodegenerative disorders, underscoring its importance in neuronal iron management.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 700–963 of human IRP2, a region that may encompass part of the RNA-binding domain. For applications requiring different epitopes, consider antibodies targeting the N-terminal region.
• This antibody has been validated for Western blot (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), and ELISA. In WB, a specific band around 105 kDa is expected. Due to preserved cross-reactivity with mouse and rat, it is suitable for comparative studies across these species.
• Optimal working concentrations should be determined empirically. Include appropriate positive and negative controls, especially when exploring tissue-specific expression or disease models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a comprehensive portfolio of IVD raw materials. For iron metabolism studies, we provide validated antibody pairs, optimized anti-IRP2 monoclonal antibodies, and bulk ancillary reagents. As your strategic sourcing partner, we also facilitate procurement of rare target raw materials and offer technical consulting to accelerate your projects from concept to clinic.