Anti-IRF8 Polyclonal Antibody for WB, ELISA - Q02556

Core Product Specifications and Parameters

Biological Background: IRF8 Function and Localization

• Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes, acting as a transcriptional activator or repressor.
  Related references: PMID:25122610
• Plays a critical role in hematopoietic cell differentiation; essential for the development of plasmacytoid dendritic cells (pDCs) and CD8+ dendritic cells via cooperative binding with BATF-JUNB at AICE motifs.
• Positively regulates macroautophagy in dendritic cells.
  Related references: PMID:29434592
• Also acts as a repressor of osteoclast differentiation by inhibiting factors such as NFATC1 and EEIG1.
• Localizes to both the nucleus and cytoplasm, with predominant expression in lymphoid tissues.
• Known to undergo ubiquitination; disease mutations linked to immunodeficiency (IMD32A, IMD32B).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the central region (aa 137-426) of human IRF8; consider epitope mapping to assess cross-reactivity with other IRF family members.
• For Western blotting, prepare nuclear extracts to enrich IRF8 due to its predominant nuclear localization.
• The predicted molecular weight is 48 kDa, but post-translational modifications may cause minor band shifts; validate the expected size in your sample system.
• When performing ELISA, optimize coating conditions and detection thresholds using recombinant IRF8 protein as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with streamlined access to high-quality IVD raw materials. This anti-IRF8 polyclonal antibody supports immunodetection studies in immune cell biology and interferon signaling. As your one-stop sourcing partner, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development.