Anti-IRF6 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - O14896

Core Product Specifications and Parameters

Biological Background: IRF6 Function and Localization

• IRF6 is a probable DNA-binding transcriptional activator that plays a key role in the keratinocyte proliferation-differentiation switch, essential for proper epidermal development.
• It is involved in the regulation of mammary epithelial cell proliferation.
• IRF6 may regulate the transcription of WDR65.
• Subcellular localization is primarily nuclear, but it can also be found in the cytoplasm, reflecting dynamic shuttling.
• Expression is high in normal mammary epithelial cells but is significantly reduced or absent in breast carcinomas, suggesting a tumor-suppressive role.
• The protein undergoes alternative splicing and is subject to ubiquitin-like conjugation (Ubl conjugation), indicating post-translational regulation.
• Mutations in IRF6 are associated with disease variants, including popliteal pterygium syndrome and Van der Woude syndrome (reflected in aliases: PPS, VWS).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 368–467) of human IRF6, which may confer specificity for certain isoforms or species variants; validate in the intended sample system.
• For Western blot (WB), a band at approximately 53 kDa is expected under standard denaturing conditions.
• In immunofluorescence/immunocytochemistry (IF/ICC), both nuclear and cytoplasmic staining may be observed depending on cell type and activation state.
• For immunoprecipitation (IP), the antibody may capture endogenous IRF6 from human, mouse, or rat lysates; optimize antibody-to-lysate ratios.
• ELISA performance should be validated using recombinant IRF6 or the peptide immunogen as a positive control.
• When analyzing breast tissue samples, note that IRF6 expression is often downregulated in carcinomas; include positive and negative controls for accurate interpretation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated monoclonal antibodies like this IRF6 Rabbit mAb. As a B2B sourcing partner, we offer bulk ancillary reagents and support rare target raw-material procurement to accelerate your epidermal differentiation or breast cancer research programs. Contact us to explore our comprehensive portfolio tailored for your diagnostic development needs.