Anti-IRF5 Rabbit Polyclonal Antibody for WB - Q13568

Core Product Specifications and Parameters

Biological Background: IRF5 Function and Localization

• IRF5 (Interferon regulatory factor 5) is a transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN-α/IFN-β) and inflammatory cytokines. Related references: PMID:11303025 PMID:15695821 PMID:22412986
• It mediates IFN induction downstream of endolysosomal toll-like receptors TLR7, TLR8, and TLR9 via the MyD88-dependent signaling pathway.
• IRF5 is a key transcription factor regulating the IFN response during SARS-CoV-2 infection. Related references: PMID:33440148
• The protein binds to interferon-stimulated response elements (ISRE) in the promoters of target genes and can efficiently activate both IFN-beta and IFN-alpha genes.
• IRF5 localizes to both the cytoplasm and nucleus and is known to be phosphorylated and ubiquitinated as part of its activation mechanism.
• Alternative splicing of IRF5 generates multiple isoforms, and polymorphisms in the IRF5 gene are associated with systemic lupus erythematosus (SLE).
• IRF5 is involved in antiviral defense, DNA-binding, inflammatory response, and is subject to phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-200) of human IRF5, which may result in detection of multiple isoforms if this region is conserved across splice variants.
• For Western blotting, a band at approximately 56 kDa is expected; consider using positive control lysates from human or rat cells treated with TLR agonists.
• For immunohistochemistry, validate antibody specificity by using appropriate tissue controls and considering the reported nuclear and cytoplasmic localization.
• In ELISA applications, this polyclonal antibody can be used as a capture or detection reagent; pairing with a monoclonal antibody may improve assay specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide comprehensive access to IVD raw materials. For innate immunity targets like IRF5, we offer validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents to support your assay development from concept to clinic.