Anti-IRF5 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q13568

Core Product Specifications and Parameters

Biological Background: IRF5 Function and Localization

• Interferon regulatory factor 5 (IRF5) is a transcription factor encoded by the IRF5 gene (also known as SLEB10). It belongs to the IRF family and contains a DNA-binding domain that recognizes interferon-stimulated response elements (ISRE).
• IRF5 functions as a central regulator of innate immunity, activating the expression of type I interferons (IFN-α and IFN-β) and inflammatory cytokines in response to endosomal Toll-like receptors TLR7, TLR8, and TLR9. Related references: PMID:11303025 PMID:15695821 PMID:22412986
• It drives transcription of IFN-stimulated genes (ISGs) by binding to ISRE in their promoters, thereby amplifying antiviral responses.
• IRF5 is critical for the interferon response during SARS-CoV-2 infection, as it regulates the expression of antiviral effectors. Related references: PMID:33440148
• Subcellular localization of IRF5 is dynamically regulated; it shuttles between the cytoplasm and nucleus, with nuclear translocation being essential for its transcriptional activity.
• Post-translational modifications, including phosphorylation, ubiquitination (isopeptide bond), and ubiquitin-like conjugation, modulate IRF5 stability and activity.
• Alternative splicing generates multiple isoforms, contributing to functional diversity and tissue-specific regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal DNA-binding domain (aa 6-151) of human IRF5, suggesting that the antibody may recognize this functional region; consider this when designing epitope-mapping experiments.
• For Western blotting, the predicted molecular weight of IRF5 is approximately 56 kDa; ensure adequate separation and use appropriate controls to confirm band identity.
• IRF5 expression is inducible upon TLR activation in immune cells; consider treating cell lines (e.g., THP-1, RAW 264.7) with TLR agonists to enhance detection.
• Given the reported cross-reactivity with mouse, this antibody may be suitable for mouse models; however, validate performance in the relevant tissue or cell type.
• For immunoprecipitation (IP), verify the antibody's pulldown efficiency in overexpressed or endogenous systems, as IRF5 may interact with other proteins.
• In ELISA, a matched antibody pair may be required; consider pairing with a detection antibody targeting a non-overlapping epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials for immunology and infectious disease research, including validated IRF5 antibody pairs and optimized monoclonal antibodies like this IRF5 Rabbit mAb. Beyond antibodies, we supply bulk ancillary reagents and can source rare-target raw materials to accelerate your assay development from concept to clinic.