Anti-Irf3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P70671

Core Product Specifications and Parameters

Biological Background: Irf3 Function and Localization

• Interferon regulatory factor 3 (Irf3) is a key transcriptional regulator of type I interferon (IFN)-dependent innate immune responses, playing a critical role in antiviral defense against DNA and RNA viruses. It regulates transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to interferon-stimulated response elements (ISRE) in their promoters, acting as a more potent activator of IFNB than IFNA. Related references:
  • PMID:15800576
  • PMID:39719712
• In uninfected cells, Irf3 exists as an inactive cytoplasmic monomer. Upon viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, it is phosphorylated by IKBKE and TBK1 kinases, inducing a conformational change that leads to dimerization, nuclear translocation, and association with CREBBP to form the DRAF1 complex, which activates transcription of type I IFN and ISG genes. Irf3 can also induce apoptosis in primary macrophages. Related references:
  • PMID:16846591
  • PMID:16979567
  • PMID:20049431
• Alternative splicing generates multiple Irf3 isoforms; one isoform may act as a modulator, decreasing the transcriptional activity of isoform 1 by competing for ISRE binding.
• Subcellular localization of Irf3 includes cytoplasm, nucleus, and mitochondria, reflecting its dynamic shuttling upon activation.
• Key post-translational modifications include phosphorylation, acetylation, and ubiquitination (including isopeptide bond and Ubl conjugation), which regulate its activity, stability, and interactions.
• Irf3 is widely expressed across tissues, consistent with its broad role in innate immunity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-200 of mouse Irf3; this central region is likely conserved across species, supporting observed cross-reactivity with human and rat. Consider sequence alignment to verify epitope conservation if using in other species.
• For Western blotting, the predicted molecular weight is 46 kDa. Use appropriate positive and negative controls, such as stimulated vs. unstimulated cell lysates, to confirm specificity.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution for each tissue type. Detection may be enhanced in tissues with activated immune responses.
• For ELISA, validate the antibody with recombinant Irf3 protein or known positive samples. Pair with an appropriate detection antibody for sandwich ELISA development.
• Given Irf3's role in innate immunity, consider stimulating cells with viral mimics (e.g., poly(I:C)) or interferons to upregulate target expression for improved detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing IVD manufacturers, research laboratories, and diagnostic developers with a comprehensive range of raw materials, technical services, and consulting support from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-Irf3 polyclonal antibody is positioned to support research in innate immunity and interferon signaling, and we can assist with scaling and custom conjugation needs.