Anti-IRF3 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA, ChIP - Q14653

Core Product Specifications and Parameters

Biological Background: IRF3 Function and Localization

• IRF3 is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses, playing a critical role in innate immunity against DNA and RNA viruses. Related references: PMID:22394562, PMID:24049179, PMID:25636800
• It regulates transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Related references: PMID:11846977, PMID:16846591, PMID:16979567
• IRF3 is a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Related references: PMID:16846591, PMID:16979567, PMID:20049431
• Found in an inactive form in the cytoplasm of uninfected cells; following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, it is phosphorylated by IKBKE and TBK1 kinases, inducing a conformational change that leads to dimerization and nuclear localization. Related references: PMID:22394562, PMID:25636800, PMID:27302953
• In the nucleus, IRF3 associates with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex that activates the transcription of type I IFN and ISG genes. Related references: PMID:16154084, PMID:27302953, PMID:33440148
• In response to Sendai virus infection, IRF3 is recruited to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX, inducing apoptosis. Related references: PMID:25609812
• Expressed constitutively in a variety of tissues.
• Post-translational modifications include phosphorylation (critical for activation), acetylation, and ubiquitination; alternative splicing generates additional isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–200 of human IRF3 (NP_001184051.1), a region within the N-terminal DNA-binding domain; consider using this region for epitope mapping or blocking experiments.
• In Western blot, a band at approximately 47 kDa is expected; validate in relevant virus-infected or IFN-stimulated cell lines to confirm expression.
• Cross-reactivity with mouse and rat IRF3 is reported; verify sequence homology in the immunogen region if using these species.
• For ChIP applications, optimize cross-linking and sonication conditions due to IRF3's dynamic nucleocytoplasmic shuttling.
• For IF/ICC, use appropriate fixation and permeabilization methods to detect both cytoplasmic and nuclear localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, serving diagnostic manufacturers, laboratories, and research institutes. Our catalog includes validated polyclonal and monoclonal antibodies, such as this anti-IRF3 pAb, along with recombinant proteins and ancillary reagents tailored for innate immunity and antiviral research. We also provide bulk supply and custom sourcing for rare targets to accelerate your assay development from concept to clinic.