Anti-IRF3 (IIAE7) Rabbit Polyclonal Antibody for WB and ELISA - Q764M6

Core Product Specifications and Parameters

Biological Background: IRF3 Function and Localization

• IRF3 is a key transcriptional regulator of type I interferon-dependent immune responses, playing a critical role in innate immunity against DNA and RNA viruses.
• It regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters.
• In uninfected cells, IRF3 is found in an inactive form in the cytoplasm; upon viral infection or TLR signaling, it is phosphorylated by IKBKE and TBK1 kinases, leading to dimerization and nuclear translocation.
• In the nucleus, IRF3 associates with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex that activates transcription of type I IFN and ISG genes.
• IRF3 can activate distinct gene expression programs in macrophages and induce significant apoptosis in primary macrophages.
• IRF3 localizes to the cytoplasm, nucleus, and mitochondrion, and is subject to post-translational modifications including phosphorylation, acetylation, and ubiquitination, as indicated by keywords.
• It is involved in antiviral defense and host-virus interactions, and contains a DNA-binding domain critical for transcriptional activation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within the N-terminal 1-100 amino acids of Sus scrofa IRF3; cross-reactivity with human IRF3 is reported. Verify reactivity in your specific sample system.
• For Western blot (WB), consider using whole cell lysates from human or pig cells, and validate antibody specificity with appropriate knockdown or overexpression controls. The predicted molecular weight is 47 kDa, though observed migration may vary.
• For ELISA, consider coating with the immunogen peptide or recombinant IRF3 protein; perform a titration to determine the optimal antibody concentration.
• As a polyclonal antibody, it may recognize multiple epitopes, potentially enhancing sensitivity for detection applications.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to critical reagents like this anti-IRF3 antibody. Leveraging our one-stop supply chain, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets in innate immunity and antiviral research. Contact us to streamline your procurement from concept to clinic.