Anti-IRF2BP2 Polyclonal Antibody for WB, ELISA - Q7Z5L9

Core Product Specifications and Parameters

Biological Background: IRF2BP2 Function and Localization

• IRF2BP2 (Interferon regulatory factor 2-binding protein 2) functions as a transcriptional corepressor in an IRF2-dependent manner, and its repressive activity is independent of histone deacetylase.
  Related references: PMID:12799427
• It represses NFAT1-dependent transactivation of NFAT-responsive promoters, linking it to immune signaling pathways.
  Related references: PMID:21576369
• Interestingly, IRF2BP2 acts as a coactivator of VEGFA expression in cardiac and skeletal muscles, demonstrating context-dependent regulatory roles.
  Related references: PMID:20702774
• The protein plays a role in immature B-cell differentiation, and mutations are associated with common variable immunodeficiency 14 (CVID14).
  Related references: PMID:27016798
• IRF2BP2 localizes to both the cytoplasm and nucleus, consistent with its roles in signal transduction and transcriptional regulation.
• It contains a zinc-finger domain and undergoes post-translational modifications including acetylation and phosphorylation, as indicated by Proteomics identification.
• The gene undergoes alternative splicing, producing multiple isoforms; the canonical isoform has a molecular weight of approximately 61 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 475-545 near the C-terminus of human IRF2BP2. Consider this region for epitope specificity when designing blocking peptides or verifying cross-reactivity.
• This antibody is recommended for Western blot (WB) and ELISA. For WB, a band around 61 kDa is expected; validate in the relevant sample system using appropriate loading controls.
• Given nuclear and cytoplasmic localization, consider subcellular fractionation or immunofluorescence (not listed in applications but may be possible with optimization) if exploring localization studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting across every stage from concept to clinic. This anti-IRF2BP2 polyclonal antibody supports research in immunology, transcriptional regulation, and B-cell biology. We can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials tailored to your developmental needs.