Anti-IRF2 Rabbit Monoclonal Antibody for WB, IP, ELISA - P14316

Core Product Specifications and Parameters

Biological Background: IRF2 Function and Localization

• IRF2 is a DNA-binding transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible genes, primarily functioning as a transcriptional repressor. Related references: PMID:39013473 PMID:7687740
• It also acts as a transcriptional activator for several genes, including H4 and IL7, through direct promoter binding and activation during cell growth. Related references: PMID:15226432 PMID:9540062
• IRF2 antagonizes IRF1-mediated transcriptional activation, playing a key role in the balance of interferon signaling.
• The protein localizes to the nucleus and associates with chromosomes, consistent with its role in gene regulation.
• IRF2 is expressed throughout the epithelium of the colon and in the lamina propria, suggesting tissue-specific regulatory functions.
• Multiple post-translational modifications have been reported, including phosphorylation, acetylation, and ubiquitination, which may modulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 250–349 of human IRF2, targeting a region that is conserved in mouse, supporting cross-reactivity.
• For Western blotting, consider using whole-cell lysates from human or mouse cell lines; the predicted molecular weight is ~39 kDa.
• In immunoprecipitation experiments, validate the antibody’s efficiency with a known interaction partner or co-precipitation control.
• For ELISA, ensure appropriate blocking and coating conditions; consider using a recombinant IRF2 protein as a positive control if available.
• Because IRF2 can be regulated by post-translational modifications, treatment with relevant inducers (e.g., interferon) may alter detection signals—optimize sample preparation accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated sourcing partner for IVD raw materials, offering seamless access to validated antibodies, bulk reagents, and rare-target monoclonals. For transcription factor and interferon signaling research, we supply high-quality IRF2 antibodies and related reagents to support your assay development from concept to clinic. Whether you need optimized antibody pairs, bulk ancillary materials, or sourcing for challenging targets, CamelBio streamlines procurement with a comprehensive B2B supply model.