Anti-IRF1 Rabbit Polyclonal Antibody for WB, ELISA - P15314

Core Product Specifications and Parameters

Biological Background: IRF1 Function and Localization

• IRF1 is a transcriptional regulator with diverse functions in cellular responses such as host defense, hematopoiesis, inflammation, and cell growth control. Related references: PMID:11244049, PMID:11846971, PMID:11846974
• It binds to ISRE sequences to modulate expression of genes involved in antiviral, antibacterial, anti-proliferative, apoptotic, and immune pathways. Related references: PMID:12387893, PMID:17018642, PMID:18955028
• It also represses genes related to cell cycle progression and immune tolerance, including BIRC5, cyclins, CDKs, FOXP3, and TLR4. Related references: PMID:18641303
• IRF1 localizes to both the nucleus and cytoplasm, consistent with its transcriptional and extranuclear regulatory functions.
• Post-translational modifications, including acetylation, phosphorylation, and ubiquitin-like conjugation, modulate IRF1 activity and stability.
• IRF1 is critical for immune cell development, influencing NK cell activity, macrophage IL-12 production, Th1 differentiation, and CD8+ T-cell maturation. Related references: PMID:11244049, PMID:11846971, PMID:11846974
• It functions as a tumor suppressor, antagonizing tumor growth and promoting anti-tumor immune responses. Related references: PMID:11244049, PMID:11846971, PMID:11846974

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 113-212) of human IRF1, which shares sequence similarity with mouse IRF1 (confirmed cross-reactivity). For western blot, expect a band near 37 kDa in mouse samples; consider optimizing exposure times to capture both basal and induced signals.
• For ELISA applications, validate antibody coating and dilution factors using a recombinant IRF1 protein or cell lysates as a positive control; a secondary detection system should be optimized accordingly.
• Due to the polyclonal nature, lot-to-lot consistency should be verified when used in quantitative assays; pilot experiments are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes by providing a comprehensive range of IVD raw materials, technical services, and consulting—from concept to clinical application. This anti-IRF1 antibody targets a key transcriptional regulator involved in immunity, inflammation, and oncology, making it suitable for development of research and diagnostic kits. Beyond single antibodies, CamelBio offers validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and sourcing of rare-target raw materials to streamline your IVD development pipeline.