Anti-IRF1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P10914

Core Product Specifications and Parameters

Biological Background: IRF1 Function and Localization

• IRF1 is a member of the interferon regulatory factor family of transcription factors. Related references: PMID:15226432 PMID:15509808 PMID:17516545
• It functions as a transcriptional activator and repressor, regulating genes involved in antiviral and antibacterial responses, inflammation, cell proliferation, and apoptosis by binding to interferon-stimulated response elements (ISRE). Related references: PMID:15226432 PMID:15509808 PMID:17516545 PMID:18641303 PMID:22200613
• IRF1 plays a crucial role in innate and adaptive immunity, including NK cell maturation, macrophage IL12 production, Th1 development, and CD8+ T-cell differentiation. Related references: PMID:11244049 PMID:11846971 PMID:11846974
• It is essential for IFNG-dependent immunity to mycobacteria. Related references: PMID:36736301
• IRF1 acts as a tumor suppressor by regulating p53-dependent transcription and inducing growth arrest and apoptosis. Related references: PMID:15509808 PMID:18084608 PMID:20049431
• It localizes predominantly to the nucleus but can also be found in the cytoplasm.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide between amino acids 181-280 of human IRF1. The antibody may recognize full-length IRF1 and possibly its isoforms or post-translationally modified forms.
• For Western blotting, a band at approximately 37 kDa is expected; however, actual migration may vary due to modifications. Validate using relevant positive and negative controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), confirm specificity through pre-adsorption with the immunizing peptide and by comparing staining patterns with known expression data.
• The antibody is predicted to cross-react with mouse and rat IRF1; however, we recommend verifying reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, connecting diagnostic manufacturers, labs, and research institutes with high-quality reagents and technical support from concept to clinic. This anti-IRF1 polyclonal antibody represents our comprehensive portfolio for immunology and infectious disease research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—ensuring a reliable supply chain for your diagnostic development needs.