Anti-IRE1 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - O75460

Core Product Specifications and Parameters

Biological Background: IRE1 Function and Localization

• IRE1 (ERN1) is a serine/threonine-protein kinase and endoribonuclease that serves as a critical sensor for the unfolded protein response (UPR) in the endoplasmic reticulum (ER). In unstressed cells, its luminal domain is bound by the chaperone HSPA5/BiP; upon accumulation of misfolded proteins, HSPA5/BiP dissociates, allowing IRE1 to homodimerize, autophosphorylate, and activate its endoribonuclease domain. Related references: PMID:11175748, PMID:11779464, PMID:12637535, PMID:21317875

• The activated endoribonuclease specifically cleaves XBP1 mRNA, excising a 26-nucleotide intron to generate a spliced transcript that encodes a potent transcriptional activator driving expression of UPR target genes. Related references: PMID:11779464, PMID:21317875, PMID:24508390

• IRE1 also participates in ER stress-induced unconventional trafficking of CFTR to the cell membrane by modulating the expression and localization of SEC16A. Related references: PMID:21884936, PMID:28067262

• IRE1 is a single-pass type I membrane protein predominantly located in the endoplasmic reticulum membrane.

• It is ubiquitously expressed in human tissues, with high levels observed in the pancreas.

• The protein undergoes multiple post-translational modifications, including autophosphorylation, ADP-ribosylation, glycosylation, and disulfide bond formation, which regulate its activity.

• The full-length protein has a calculated molecular weight of approximately 110 kDa (UniProt: 109.7 kDa; supplier reported 110 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 286–435 in the cytoplasmic region of human IRE1, which includes part of the kinase domain. This region is well-conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blot (WB), a band at approximately 110 kDa is expected; use reducing conditions and validate with appropriate loading controls and ER stress inducers (e.g., tunicamycin, thapsigargin) to confirm specificity.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), the protein is localized to the endoplasmic reticulum; permeabilization is required, and co-staining with ER markers may aid validation.
• For ELISA applications, consider using the antibody as a capture or detection reagent and validate performance with recombinant IRE1 or cell lysates from treated vs. untreated samples.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio supports diagnostic manufacturers and research laboratories with validated antibodies like this anti-IRE1 polyclonal antibody, targeting the unfolded protein response pathway. We offer bulk supply, custom antibody development, and complementary reagents—including matched pairs, recombinant proteins, and ancillary assay components—to streamline your ER stress-related assay development and biomarker programs.