Anti-IRE1 Monoclonal Antibody for WB, ELISA - O75460

Core Product Specifications and Parameters

Biological Background: IRE1 Function and Localization

• IRE1 (also known as IRE1P, IRE1a, or hIRE1p) is a ~110 kDa serine/threonine-protein kinase and endoribonuclease encoded by the ERN1 gene in humans.
• IRE1 serves as a critical sensor in the endoplasmic reticulum (ER) unfolded protein response (UPR). Under basal conditions, its luminal domain is kept inactive by binding to HSPA5/BiP. Accumulation of misfolded proteins triggers release, homodimerization, autophosphorylation, and activation of its endoribonuclease domain. Related references: PMID:11175748, PMID:11779464, PMID:12637535, PMID:21317875
• The activated endoribonuclease domain specifically cleaves 26 nucleotides from XBP1 mRNA, generating a spliced transcript that encodes a potent transcription factor driving UPR target gene expression. Related references: PMID:11779464, PMID:21317875, PMID:24508390
• IRE1 is an ER-resident transmembrane protein, with both its kinase and RNase domains facing the cytoplasm, and it functions as a proximal signal for ER stress-mediated unconventional trafficking pathways, such as CFTR delivery to the plasma membrane via GORASP2 and SEC16A modulation. Related references: PMID:21884936, PMID:28067262
• The protein undergoes multiple post-translational modifications including phosphorylation, ADP-ribosylation, and glycosylation, and harbors key functional motifs: an ATP-binding kinase domain, a disulfide bond-stabilized luminal sensor, and a metallo-dependent endoribonuclease active site.
• IRE1 is ubiquitously expressed in human tissues, with particularly high levels in the pancreas, consistent with its central role in ER proteostasis.
• Alternatively spliced isoforms and polymorphisms of ERN1 may fine-tune UPR signaling intensity and contribute to cell fate decisions between adaptation and apoptosis.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant fusion protein covering amino acids 286–435 of human IRE1 (NP_001424.3), which lies within the juxtamembrane and kinase domain regions. This region is conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blot applications, consider using ER stress inducers (e.g., tunicamycin, thapsigargin) to enhance IRE1 detection, and validate the antibody in the relevant sample system with appropriate loading controls.
• In ELISA, optimize coating and detection conditions as the antibody recognizes a linear epitope within the immunogen sequence.
• The predicted molecular weight is ~110 kDa, but post-translational modifications and activation-induced phosphorylation may cause slight mobility shifts; confirm with molecular weight markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials like this IRE1 monoclonal antibody, which targets the ER stress and UPR pathway—a critical area in cancer, neurodegenerative diseases, and metabolic disorders. As a dedicated B2B sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target raw-material procurement, all backed by technical and supply chain support to accelerate your assay development from concept to clinic.