Anti-IRAK4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NWZ3

Core Product Specifications and Parameters

Biological Background: IRAK4 Function and Localization

• IRAK4 is a serine/threonine-protein kinase critical for initiating innate immune responses, specifically involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Related references: PMID:17878374
• Upon TLR activation, IRAK4 is rapidly recruited by MYD88 to form the Myddosome complex with IRAK2, leading to phosphorylation and subsequent autophosphorylation of IRAK1.
• Phosphorylates Pellino E3 ubiquitin ligases (PELI1, PELI2, PELI3) to promote polyubiquitination of IRAK1, ultimately facilitating NF-kappa-B nuclear translocation and activation.
• Alternatively, phosphorylates TIRAP to promote its ubiquitination and degradation, and also phosphorylates NCF1, regulating NADPH oxidase activation after LPS stimulation.
• Primarily localizes in the cytoplasm, consistent with its role in signal transduction from membrane receptors.
• Belongs to the protein kinase superfamily and contains functional domains for ATP binding and magnesium coordination; subject to acetylation and phosphorylation; alternative splicing reported; mutations are implicated in primary immunodeficiency disorders.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1–220) of IRAK4, potentially recognizing full-length and N-terminal isoforms; verify in your specific sample system.
• For Western blot (WB), expect a band near 52 kDa; include proper controls to confirm specificity.
• In IHC and IF/ICC, standard antigen retrieval and blocking protocols are suggested; cytoplasmic staining should be observed.
• ELISA performance can be optimized with recombinant IRAK4 or lysates; consider paired antibody formats for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement by providing validated antibodies, recombinant proteins, and ancillary reagents for diagnostic and research applications. For innate immunity and kinase signaling targets like IRAK4, we offer one‑stop access to optimized polyclonals, matched antibody pairs, and bulk raw materials. Partner with CamelBio to accelerate your assay development from concept to clinic.