Anti-IRAK4 Rabbit Monoclonal Antibody for WB, ELISA - Q9NWZ3

Core Product Specifications and Parameters

Biological Background: IRAK4 Function and Localization

• Interleukin-1 receptor-associated kinase 4 (IRAK4), also known as renal carcinoma antigen NY-REN-64, is encoded by the IRAK4 gene in humans.
• Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens; involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Related references: PMID:17878374
• Upon TLR activation, IRAK4 is rapidly recruited by MYD88 to form the Myddosome complex together with IRAK2, where it phosphorylates and stimulates IRAK1 kinase activity.
• Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2, PELI3) to promote IRAK1 polyubiquitination, enabling IKBKG/NEMO binding and subsequent NF-kappa-B activation via the IKK complex.
• Additionally, phosphorylates TIRAP to promote its ubiquitination and degradation, and regulates NADPH oxidase activation by phosphorylating NCF1.
• Subcellular location is cytoplasmic.
• Alternative splicing produces multiple isoforms; the protein is associated with disease variants such as immunodeficiency (IMD67) and contains kinase-essential motifs (ATP-binding, magnesium, nucleotide-binding).

Experimental Guidance and Technical Tips

• The immunogen (N-terminal amino acids 1-200) spans part of the kinase domain; the antibody may detect total IRAK4 protein with a predicted molecular weight of ~52 kDa.
• KD-validated for Western blot, this antibody can be used to confirm knockdown efficiency or detect endogenous IRAK4 in human cell lysates.
• For ELISA applications, direct or capture formats may be feasible; users should optimize coating conditions and validate in relevant sample systems.
• Consider using human immune cell lines (e.g., THP-1, Jurkat) stimulated with TLR agonists to induce IRAK4 expression and assess pathway activation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with integrated access to high-quality IVD raw materials, including validated monoclonal antibodies like anti-IRAK4 for innate immunity studies. As a sourcing partner, we support your projects from concept to clinic with tailored antibody pairs, bulk ancillary reagents, and expertise in rare target procurement—streamlining your development of immunoassays and signaling pathway research.