Anti-IRAK3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y616

Core Product Specifications and Parameters

Biological Background: IRAK3 Function and Localization

• IRAK3, also known as IRAK-M, is a member of the interleukin-1 receptor-associated kinase (IRAK) family and functions as an inactive pseudokinase due to lack of catalytic activity, though it retains an ATP-binding pocket.
• Acts as a negative regulator of innate immune signaling by suppressing the dissociation of IRAK1 and IRAK4 from the Toll-like receptor (TLR)/IL-1R complex, thereby dampening downstream NF-κB and MAPK pathway activation (PubMed:10383454).
  Related references: PMID:10383454
• In response to IL-33 stimulation in lung dendritic cells, IRAK3 promotes the expression of IL-6, G-CSF, CXCL2, and CCL5, contributing to eosinophilic airway inflammation (PubMed:29686383).
  Related references: PMID:29686383
• Localizes to both the cytoplasm and nucleus, suggesting potential shuttling or dual compartment functions in signal transduction.
• Predominantly expressed in peripheral blood lymphocytes, and at the protein level in eosinophils, dendritic cells, and monocytes (PubMed:10383454, PubMed:29686383).
  Related references: PMID:10383454 PMID:29686383
• Contains multiple alternative splice isoforms and is reported as a phosphoprotein with potential disulfide bonds. Keywords include asthma, highlighting its relevance in allergic and inflammatory diseases.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 367-596) encompassing part of the kinase-like domain and the C-terminal tail. This design may capture multiple IRAK3 isoforms, but isoform specificity should be verified in the relevant model system.
• For Western blot (WB), a band at approximately 68 kDa is predicted. Consider using whole-cell lysates from stimulated human peripheral blood mononuclear cells (PBMCs), mouse macrophages, or rat dendritic cells as positive controls.
• For immunofluorescence/immunocytochemistry (IF/ICC), expect cytoplasmic and/or nuclear staining. Fixation and permeabilization conditions should be optimized for immune cell types.
• For ELISA assays, the antibody can be used as a capture or detection reagent; however, pairing with a compatible antibody and optimizing the assay conditions are recommended.
• Given the cross-reactivity with mouse and rat, validate the antibody in the specific species and tissue of interest, as signal intensity may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a comprehensive portfolio of IVD raw materials and technical services to accelerate development from concept to clinic. Our IRAK3 polyclonal antibody is part of an expanding immunology and inflammation reagent collection. In addition to validated antibodies, we provide optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare or emerging targets. Contact us to tailor procurement solutions that match your project’s scale and compliance needs.