Anti-IRAK2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O43187

Core Product Specifications and Parameters

Biological Background: IRAK2 Function and Localization

• IRAK2 (Interleukin-1 receptor-associated kinase-like 2) is a member of the interleukin-1 receptor-associated kinase family, featuring a protein kinase-like domain.
• Upon IL-1 stimulation, IRAK2 binds to the IL-1 type I receptor, triggering intracellular signaling cascades that drive transcriptional upregulation and mRNA stabilization.
• It is broadly expressed in immune-related and other tissues, including spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas, and peripheral blood leukocytes.
• The protein contains ATP-binding and nucleotide-binding motifs, indicative of a kinase fold, and is classified as a phosphoprotein.
• A three-dimensional structure is available, facilitating structural and functional analyses of the IRAK2 signaling complex.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 50–250 of human IRAK2; targeting this region may be useful for epitope-specific assays.
• In Western blotting, expect a band at approximately 69 kDa; use molecular weight markers to confirm specificity.
• For immunohistochemical staining, spleen and thymus sections are recommended as positive controls due to high IRAK2 expression.
• Although cross-reactivity with rat is indicated, verify performance in your specific model system to ensure reliable results.
• For ELISA, titrate the antibody and optimize coating/buffer conditions; consider pairing with a suitable detection antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with comprehensive access to IVD raw materials, from validated antibody pairs to rare-target reagents. For IRAK2 research—a key component of IL-1 signaling and inflammation pathways—CamelBio offers optimized monoclonal antibodies, bulk ancillary reagents, and tailored sourcing support to accelerate your assay development from concept to clinic.