Anti-IRAK1 Rabbit Monoclonal Antibody for WB, ELISA - P51617

Core Product Specifications and Parameters

Biological Background: IRAK1 Function and Localization

• IRAK1 (Interleukin-1 receptor-associated kinase 1) is a serine/threonine-protein kinase essential for initiating innate immune responses; it exists as two ubiquitously expressed isoforms, with isoform 1 being more abundant.
• IRAK1 is a critical component of Toll-like receptor (TLR) and IL-1R signaling pathways, rapidly recruited by MYD88 upon activation, leading to phosphorylation by IRAK4 and subsequent autophosphorylation.
• Activated IRAK1 phosphorylates Pellino proteins (PELI1, PELI2, PELI3), promoting polyubiquitination of IRAK1, which scaffolds the IKBKG/NEMO-IKK complex for NF-kappa-B nuclear translocation.
• Additionally, IRAK1 phosphorylates TIRAP to promote its degradation and phosphorylates IRF7 to drive type I IFN gene expression, establishing an antiviral state.
• Upon sumoylation, IRAK1 translocates to the nucleus and phosphorylates STAT3, indicating a non-canonical role in transcriptional regulation.
• Subcellularly, IRAK1 localizes to the cytoplasm, nucleus, and lipid droplets, reflecting its dynamic involvement in multiple signaling compartments.
• IRAK1 is characterized by keywords including ATP-binding, kinase, phosphoprotein, isopeptide bond, and host-virus interaction, highlighting its regulatory versatility.

Experimental Guidance and Technical Tips

• The immunogen (residues 215–499) covers the kinase domain; the antibody likely recognizes both isoforms containing this region.
• For Western blotting, use a reduced-denaturing gel with markers around 77 kDa and include human cell lysates as positive controls.
• For ELISA, consider coating with recombinant IRAK1 protein (aa 215–499) and validate detection in human-derived samples.
• Cross-reactivity is reported only for human; verify sequence homology before using with other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a reliable one-stop sourcing solution for high-quality IVD raw materials. This anti-IRAK1 monoclonal antibody is ideal for immunology and inflammation research, and we can also supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development from concept to clinic.