Anti-IQGAP2 Polyclonal Antibody for WB, IHC-P, ELISA - Q13576

Core Product Specifications and Parameters

Biological Background: IQGAP2 Function and Localization

• IQGAP2 encodes Ras GTPase-activating-like protein IQGAP2, a scaffolding protein that interacts with small GTPases and calmodulin.
• It binds to activated CDC42 and RAC1 but does not stimulate their GTPase activity, distinguishing it from canonical GTPase-activating proteins (GAPs).
• IQGAP2 directly associates with calmodulin, a calcium-binding messenger protein, potentially linking calcium signaling to cytoskeletal dynamics.
• The protein contains multiple repeat domains, likely contributing to its scaffolding function in assembling multi-protein complexes.
• Alternative splicing produces isoforms; isoform 2 shows enhanced expression in testis, suggesting a specialized role in reproductive biology.
• IQGAP2 is subject to phosphorylation, indicating regulation by kinase signaling pathways.
• The gene belongs to the IQGAP family, which includes IQGAP1 and IQGAP3, though functional distinctions exist.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region of human IQGAP2 (amino acids 1330-1440). For Western blot, note that the full-length protein is approximately 181 kDa; use appropriate gel acrylamide percentages and transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P), consider validating the antibody in testis tissue, where isoform 2 is known to be enriched, to confirm reactivity.
• For ELISA applications, titrate the antibody using recombinant IQGAP2 protein or cell lysates to determine optimal working concentrations.
• Cross-reactivity with mouse and rat is reported; however, always verify species-specific performance in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and laboratories with a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies like this anti-IQGAP2 reagent. Targeting the IQGAP2 scaffolding protein involved in GTPase and calmodulin signaling, this antibody can be integrated into assay development or research workflows. CamelBio streamlines procurement by offering bulk supplies, ancillary reagents, and access to rare-target antibody sourcing, helping you accelerate projects from concept to clinic.