Anti-IQGAP2 Monoclonal Antibody for WB - Q13576

Core Product Specifications and Parameters

Biological Background: IQGAP2 Function and Localization

• IQGAP2 (Ras GTPase-activating-like protein IQGAP2) is a scaffold protein that binds activated CDC42 and RAC1 but does not stimulate their GTPase activity, instead associating with calmodulin.
• As a calmodulin-binding protein, IQGAP2 participates in calcium-mediated signaling pathways.
• The gene undergoes alternative splicing, producing isoforms such as isoform 2, which exhibits enhanced expression in testis.
• IQGAP2 is classified as a phosphoprotein and has been characterized by direct protein sequencing and proteomics identification.
• The protein contains repeat motifs that likely facilitate multiple protein-protein interactions.
• 3D-structure data is available for IQGAP2, supporting structural and functional studies.

Experimental Guidance and Technical Tips

• For Western blot, consider using a low-percentage SDS-PAGE gel (e.g., 6–8%) and extended transfer times due to the high molecular weight (~181 kDa) of IQGAP2.
• In IHC-P, antigen retrieval methods such as citrate buffer (pH 6.0) or Tris-EDTA (pH 9.0) may improve epitope exposure; optimization is recommended.
• For ELISA, the immunogen region (amino acids 1301–1495) provides a specific binding domain, enabling potential development of sandwich immunoassays.
• Validate antibody specificity in tissue types where IQGAP2 is expressed, particularly in testis samples or cell models with active CDC42/RAC1 signaling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for IVD raw-material sourcing, supporting diagnostic manufacturers and researchers from concept to clinic. The IQGAP2 Rabbit mAb, reactive across human, mouse, and rat, exemplifies our commitment to providing high-quality monoclonal antibodies for cell signaling research and assay development. With expertise in supplying validated antibody pairs, bulk ancillary reagents, and rare-target raw materials, CamelBio is your trusted partner for scalable and reliable solutions.