Anti-IQGAP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P46940

Core Product Specifications and Parameters

Biological Background: IQGAP1 Function and Localization

• Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Interacts with ILK to stabilize microtubules and allow caveolae insertion (By similarity).
• Binds to activated CDC42 but does not stimulate its GTPase activity. Associates with calmodulin.
• May promote neurite outgrowth (PubMed:15695813).
  Related references:
  PMID:15695813
• May contribute to cell cycle progression after DNA replication arrest (PubMed:20883816).
  Related references:
  PMID:20883816
• Localizes to the cell membrane, nucleus, cytoplasm, cell cortex, apical cell membrane, and basolateral cell membrane.
• Expressed predominantly in the placenta, lung, and kidney, with lower levels in heart, liver, skeletal muscle, and pancreas (PubMed:8051149).
  Related references:
  PMID:8051149
• Post-translational modifications include acetylation and phosphorylation; involved in host-virus interactions.

Experimental Guidance and Technical Tips

• For Western blot, the expected band is around 189 kDa. Consider using a positive control such as placenta or lung lysates where IQGAP1 is highly expressed.
• For immunohistochemistry (IHC), optimize antigen retrieval and antibody dilution for your specific tissue type. Validate specificity with appropriate controls.
• As a polyclonal antibody, it recognizes multiple epitopes and is suitable for both denaturing and native conditions. It can be used in immunoprecipitation (IP) and ELISA.
• For immunofluorescence (IF/ICC), fix and permeabilize cells appropriately, and include a negative control to confirm target specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to high-quality IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. This IQGAP1 polyclonal antibody supports research into cytoskeletal dynamics and oncogenic signaling pathways. Contact us for bulk ordering, custom conjugation, or matching antigen controls.