Anti-IQGAP1 Monoclonal Antibody for WB, IHC-P, ELISA - P46940

Core Product Specifications and Parameters

Biological Background: IQGAP1 Function and Localization

• IQGAP1 (Ras GTPase-activating-like protein) is also known as p195, SAR1, and HUMORFA01.
• Plays a crucial role in regulating actin cytoskeleton dynamics and assembly.
• Binds to activated CDC42 but does not stimulate its GTPase activity; associates with calmodulin.
• May promote neurite outgrowth (PubMed:15695813) and contribute to cell cycle progression after DNA replication arrest (PubMed:20883816). Related references: PMID:15695813 PMID:20883816
• Localizes to the cell membrane, nucleus, cytoplasm, cell cortex, and apical/basolateral membranes.
• Expressed in placenta, lung, kidney, and at lower levels in heart, liver, skeletal muscle, pancreas (PubMed:8051149). Related references: PMID:8051149
• Post-translational modifications include acetylation and phosphorylation; involved in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human IQGAP1; consider epitope accessibility in your specific assay format.
• For Western blot applications, validate the antibody with positive and negative cell lysates to confirm specificity for the ~189 kDa band.
• In IHC-P, optimize antigen retrieval conditions and include appropriate controls to ensure specific staining.
• For ELISA, consider using a peptide competition assay to confirm binding specificity.
• Cross-reactivity with mouse IQGAP1 is reported; validate in the relevant mouse model or tissue system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, serving diagnostic manufacturers and research laboratories worldwide. We offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents—streamlining your assay development from concept to clinic. This IQGAP1 rabbit mAb supports research into actin cytoskeleton regulation, cell migration, and cancer biology, and our team can assist in sourcing rare targets or scaling production for your specific diagnostic needs.