Anti-IPO9 Rabbit Polyclonal Antibody for WB and ELISA - Q96P70

Core Product Specifications and Parameters

Biological Background: IPO9 Function and Localization

• IPO9 (Importin-9), also known as RanBP9, is a nuclear transport receptor belonging to the importin beta family.
• It mediates nuclear import of cargo proteins including histones H2A, H2B, H4, proteasomes, and actin by recognizing nuclear localization signals (NLS). Related references: PMID:11823430, PMID:30855230, PMID:34711951
• IPO9 functions as a chaperone for histones, preventing inappropriate non-nucleosomal interactions prior to nuclear import. Related references: PMID:30855230
• The protein localizes to both the cytoplasm and nucleus, shuttling between these compartments as part of its transport cycle.
• It plays a role in the nuclear import of pre-assembled proteasomes, with AKIRIN2 serving as a molecular bridge. Related references: PMID:11823430, PMID:34711951
• Post-translational modifications include acetylation; the protein has been identified through direct protein sequencing and proteomics.
• The import cycle is Ran-dependent, with directionality conferred by the asymmetric distribution of GTP- and GDP-bound Ran across the nuclear envelope. Related references: PMID:11823430

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 827–1041) of human IPO9, which may generate antibodies recognizing both full-length and truncated forms. Consider this when interpreting banding patterns in western blot.
• For WB, the predicted molecular weight is ~116 kDa. Ensure appropriate gel percentage and transfer conditions for high molecular weight proteins.
• For ELISA, the antibody may be used as a capture or detection reagent; however, optimal dilutions should be determined experimentally.
• Cross-reactivity with mouse has been reported; validate in the relevant sample system if using mouse models.
• Given IPO9's dual cytoplasmic and nuclear localization, fractionation experiments may help confirm target specificity.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop sourcing partner for IVD raw materials, CamelBio provides this IPO9 rabbit polyclonal antibody alongside a comprehensive portfolio of validated antibodies, reagents, and consultative support for diagnostic development. Whether you require optimized antibody pairs, bulk quantities, or assistance with rare target sourcing, our team is positioned to help streamline your nucleocytoplasmic transport and chaperone research applications. Contact us to explore customized supply solutions for your next project.