Anti-IPO8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O15397

Core Product Specifications and Parameters

Biological Background: IPO8 Function and Localization

• Importin-8 (IPO8), also known as Ran-binding protein 8 (RanBP8), is a member of the importin-beta family of nuclear transport receptors.
• Functions in nuclear protein import either as an autonomous nuclear transport receptor or as an adapter-like protein in complex with the importin-beta subunit KPNB1. Related references: PMID:9214382
• Mediates the nuclear import of specific cargos, including the signal recognition particle protein SRP19 in vitro. Related references: PMID:11682607
• Probable involvement in cytoplasm-to-nucleus shuttling of diverse cargos such as Argonaute-microRNA complexes, JUN, RELA/NF-kappa-B p65, EIF4E, and SMAD transcription factors.
• Subcellular localization includes both cytoplasm and nucleus, consistent with its shuttling role.
• Post-translationally regulated by phosphorylation, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein encompassing the C-terminal region (aa 878-1037) of human IPO8; consider that the antibody recognizes epitopes within this domain.
• For Western blot (WB), a band at approximately 120 kDa is expected; validate in the relevant sample system with appropriate loading controls.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect cytoplasmic and nuclear staining; permeabilization and fixation conditions should be optimized.
• Cross-reactivity is reported for human, mouse, and rat; verify in your species of interest if not listed.
• For ELISA, the antibody can be used as a capture or detection reagent after appropriate titration and pairing tests.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD raw material procurement, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This IPO8 Rabbit pAb supports research and diagnostic development in nuclear transport and importin-mediated pathways, with CamelBio's supply chain ensuring reliable access from concept to clinic.