Anti-IPO7 Polyclonal Antibody for WB, IF/ICC, ELISA - O95373

Core Product Specifications and Parameters

Biological Background: IPO7 Function and Localization

• Importin-7 (IPO7), also known as Ran-binding protein 7 (RanBP7), functions as a nuclear transport receptor that can act autonomously or as an adapter in association with importin-beta subunit KPNB1.
• Autonomously mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 via a Ran-dependent mechanism. Related references: PMID:11682607
• In association with KPNB1, facilitates the nuclear import of H1 histone, and in vitro can transport core histones H2A, H2B, H3 and H4.
• Plays a role in odontoblast differentiation by promoting nuclear translocation of transcription factors such as DLX3, KLF4 and SMAD2, and regulates MSX1 expression via SMAD1 import.
• Inhibits osteoblast differentiation by preventing RUNX2 nuclear translocation and subsequent target gene transcription.
• Subcellular localization is both cytoplasmic and nuclear, consistent with its function as a shuttling transport receptor.
• Post-translationally modified by acetylation and phosphorylation; identified by mass spectrometry in proteomic studies.
• During HIV-1 infection, Importin-7 mediates nuclear import of the viral reverse transcription complex integrase and Rev protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 900–end) of human IPO7, which may confer reactivity with isoforms containing this region.
• For western blot, a band near 120 kDa is expected; consider using reducing conditions and appropriate loading controls.
• In immunofluorescence, signals should be observable in both nucleus and cytoplasm; include appropriate controls for nuclear transport studies.
• For ELISA, optimize coating antigen and antibody concentration empirically; the polyclonal nature may reduce sensitivity compared to monoclonal alternatives.
• Cross-reactivity with mouse and rat is indicated; validate in the relevant species sample system before quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, technical support, and consulting—supporting every stage from concept to clinic. For researchers investigating nuclear transport, epigenetic regulation, or host-pathogen interactions involving importin-7, CamelBio supplies validated antibody pairs, optimized polyclonal reagents, bulk ancillary materials, and rare target sourcing. Whether you require scale-up quantities or custom logistical solutions, we bridge your project needs to high-quality supply.