Anti-IPO5 Rabbit Polyclonal Antibody for WB, ELISA - O00410

Core Product Specifications and Parameters

Biological Background: Importin-5 Function and Localization

• Importin-5 (also known as importin subunit beta-3, karyopherin beta-3, or RanBP5) is a key member of the importin beta family of nuclear transport receptors.
• Serves as a receptor for nuclear localization signals (NLS) in cargo substrates, mediating docking to the nuclear pore complex and energy-dependent, Ran-mediated translocation into the nucleus (By similarity).
• Mediates the nuclear import of ribosomal proteins RPL23A, RPS7, and RPL5, and also imports histones H2A, H2B, H3, and H4 in vitro.
  Related references:
  PMID:11682607 PMID:9687515
• Plays a role in HIV-1 infection by binding and mediating the nuclear import of HIV-1 Rev, highlighting its involvement in host-virus interactions.
• Localized predominantly in the cytoplasm, nucleus, and nucleolus, consistent with its shuttling role.
• Undergoes post-translational modifications including acetylation and phosphorylation, which may regulate its activity.
• Alternative splicing produces multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen (aa 20–300) targets the N-terminal region of IPO5, which may be effective for detecting both full-length and certain truncated forms.
• For Western blotting (WB), anticipate a band near 124 kDa; use suitable loading controls and validate with known positive and negative samples.
• In ELISA applications, the recombinant protein used as immunogen can serve as a reference standard for quantification.
• Given cross-reactivity with mouse and rat, verify reactivity in these species with appropriate tissue or cell lysates.
• Always optimize antibody dilutions and experimental conditions for your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated IVD raw material solutions, supporting research and diagnostic development from concept to clinic. This anti-IPO5 polyclonal antibody is part of our offerings for nuclear transport and cargo recognition studies. We supply validated antibody pairs, bulk ancillary reagents, and can source rare targets to streamline your assay development.