Anti-IPO4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8TEX9

Core Product Specifications and Parameters

Biological Background: IPO4 Function and Localization

• IPO4 (Importin-4), also known as Imp4b and RanBP4 (Ran-binding protein 4), is a nuclear transport receptor of the importin beta superfamily.
• It mediates the nuclear import of a broad range of classical NLS-bearing cargoes, including histones, ribosomal protein RPS3A, transition protein 2 (TNP2), and the vitamin D receptor (VDR). Related references: PMID:11823430, PMID:16207705, PMID:17682055
• Specifically transports histone H3–H4 dimers when bound to the ASF1 chaperone (ASF1A or ASF1B), as well as monomeric histones H3.1 and H4, a critical function for chromatin assembly. Related references: PMID:21454524, PMID:29408485, PMID:36103578, PMID:30177573
• In vitro, IPO4 imports the human cytomegalovirus UL84 protein by recognizing a non-classical nuclear localization signal, highlighting its versatility as a nuclear transporter. Related references: PMID:12610148
• Subcellularly, IPO4 shuttles between the cytoplasm and nucleus, with its distribution regulated by the Ran GTPase gradient across the nuclear envelope.
• Post‑translational modifications include acetylation and alternative splicing, which generate multiple isoforms (UniProt).
• Contains repetitive sequence motifs typical of nuclear transport receptors, which mediate cargo and nucleoporin interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 728–1081) of human IPO4, a segment conserved across human, mouse, and rat, explaining the antibody’s predicted cross‑reactivity with these species.
• For western blotting, the predicted molecular weight of IPO4 is approximately 119 kDa; using a 6–8% SDS‑PAGE gel and PVDF membrane is recommended for optimal resolution and transfer.
• In immunofluorescence/ICC, expect both nuclear and cytoplasmic staining; include appropriate controls (e.g., knockdown or knockout samples) to confirm specificity.
• For ELISA, the antibody can serve as a capture or detection reagent; titrate the antibody and validate with recombinant or endogenous antigen in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement by providing a comprehensive portfolio of antibodies, including this anti-IPO4 polyclonal antibody, for research on nucleocytoplasmic transport and chromatin biology. In addition to this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for challenging or rare targets. Partner with CamelBio to streamline your diagnostic development pipeline from concept to clinic.