Anti-IP3R Rabbit Polyclonal Antibody for WB, ELISA - Q14643

Core Product Specifications and Parameters

Biological Background: IP3R (ITPR1) Function and Localization

• ITPR1 encodes the inositol 1,4,5-trisphosphate receptor type 1 (IP3R1), a ligand-gated calcium channel located primarily on the endoplasmic reticulum membrane.
• Upon binding of inositol 1,4,5-trisphosphate (IP3), ITPR1 mediates calcium release from the ER into the cytoplasm, a critical step in intracellular calcium signaling (PubMed:10620513, PubMed:27108797). Related references: PMID:10620513 PMID:27108797
• The channel undergoes conformational changes to switch between closed and open states, enabling calcium ion transport across the membrane.
• ITPR1 is involved in apoptosis through activation of the CAMK2 complex and regulation of mitochondrial calcium-dependent apoptosis via interaction with HSPA9 and VDAC1.
• The protein localizes to the ER membrane, secretory vesicle membranes, and the perinuclear cytoplasm, reflecting its dynamic role in calcium storage and release.
• Widely expressed in tissues, ITPR1 also contributes to epithelial secretion, fertilization, and egg activation by modulating calcium oscillation frequency and amplitude.
• Post-translational modifications include phosphorylation, palmitoylation, and ubiquitination; the protein also features zinc-binding sites, ATP-binding capacity, and glycosylation.
• Mutations in ITPR1 are associated with spinocerebellar ataxia types 15/16 (SCA15/SCA16) and SCA29, as well as intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 1812–1911 of human IP3R (NP_001365381). This region may ensure specificity, but cross-reactivity with mouse suggests conservation; validate in your sample system.
• For WB, note the high molecular weight (~314 kDa); consider using low-percentage gels, extended transfer times, and appropriate loading controls.
• The antibody is validated for ELISA; consider direct or indirect coating methods and test serial dilutions to optimize signal-to-noise ratios.
• As cross-reactivity is reported for human and mouse, the antibody may be suitable for studies involving both species; confirm with appropriate tissue or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, technical services, and consulting—supporting development from concept to clinic. For IP3R/calcium signaling research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our network also facilitates sourcing of rare-target raw materials, enabling researchers and manufacturers to accelerate immunoassay and diagnostic kit development.