Anti-INVS Rabbit Polyclonal Antibody for WB, ELISA - Q9Y283

Core Product Specifications and Parameters

Biological Background: Inversin Function and Localization

• INVS encodes Inversin, also known as nephrocystin-2, a multifunctional protein with ANK repeats and calmodulin-binding domains.
• Critical for normal renal development and establishment of the left-right body axis during embryogenesis.
• Acts as a molecular switch between canonical and non-canonical Wnt signaling pathways by targeting cytoplasmic DVL1 for ubiquitin-proteasome degradation, thereby inhibiting canonical Wnt signaling.
• Collaborates with nephrocystin proteins NPHP1, NPHP4, and RPGRIP1L/NPHP8 to organize apical junctions in kidney tubular epithelial cells.
• Localizes to multiple subcellular compartments including the cytoplasm, cytoskeleton, mitotic spindle, membrane, nucleus, and primary cilium.
• Widely expressed in tissues with strong enrichment at the primary cilia of renal tubular cells, consistent with its role in ciliopathy-associated renal disease.
• Post-translational modifications include phosphorylation and hydroxylation, and the protein is subject to alternative splicing and ubiquitin conjugation.
• Mutations in INVS are associated with nephronophthisis type 2, an autosomal recessive cystic kidney disease.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-90); this antibody may detect full-length INVS as well as N-terminal isoforms. Validate specificity in the relevant sample system.
• For Western blot, expect a band near 118 kDa; consider using kidney-derived cell lysates (e.g., HEK293, MDCK) and optimizing loading amounts.
• In ELISA applications, use recombinant INVS protein as a positive control and perform appropriate titration experiments.
• Additional validation (e.g., siRNA knockdown, blocking peptide) is recommended to confirm antibody specificity in your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibodies for renal disease and developmental biology research. Beyond this anti-INVS polyclonal antibody, we offer matched antibody pairs, recombinant proteins, and bulk ancillary reagents to streamline your diagnostic development pipeline. Partner with CamelBio for efficient sourcing of rare-target raw materials and expert technical consultation.