Anti-Involucrin Rabbit Monoclonal Antibody for WB - P07476

Core Product Specifications and Parameters

Biological Background: Involucrin Function and Localization

• Involucrin is a major component of the cornified cell envelope (CE), an insoluble structure that forms beneath the plasma membrane of terminally differentiated keratinocytes in stratified squamous epithelia.
• The protein is rich in glutamine and lysine residues that serve as substrates for transglutaminase-mediated cross-linking, cementing the CE to intracellular structural proteins.
• Involucrin functions as an early scaffold in CE assembly, providing a template for the sequential addition of other precursors like loricrin and SPRRs.
• Encoded by the IVL gene, it belongs to a family of envelope precursor proteins that are critical for the mechanical and chemical barrier of the epidermis.
• Subcellularly, involucrin is cytoplasmic during early differentiation, becoming rapidly cross-linked at the plasma membrane during terminal keratinization.
• Expression is tightly restricted to suprabasal keratinocytes of the epidermis and other stratified squamous epithelia, including oral, esophageal, and cervical mucosa.
• Key post-translational modifications include transglutaminase-catalyzed isopeptide bonds and lipidation, both essential for envelope insolubility.
• The protein is classified under UniProt keywords: Cytoplasm, Direct protein sequencing, Isopeptide bond, Keratinization, Lipoprotein, Proteomics identification, Reference proteome, Repeat.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 100–200 of human Involucrin, a region that is likely solvent-exposed in the native protein; consider using antigen retrieval in IHC to unmask cross-linked epitopes.
• For western blot, the observed band may appear around 68 kDa, but note that involucrin can form high-molecular-weight aggregates due to cross-linking; reducing conditions and fresh lysates are recommended.
• ELISA performance may be enhanced by using a peptide-coating strategy that mirrors the linear immunogen; pair with a detection antibody recognizing a distinct epitope if developing a sandwich assay.
• Cross-reactivity with rat is observed; validate in the intended tissue and species context, as epitope conservation may vary.
• In IHC-P, involucrin staining is expected in the upper spinous and granular layers of epidermis; use appropriate positive (skin, oral mucosa) and negative (non-keratinizing epithelium) controls.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted IVD raw material partner, CamelBio streamlines your procurement of antibodies and ancillary reagents across the entire diagnostic development pipeline—from concept to commercial kit. This anti-Involucrin rabbit monoclonal antibody supports research in dermatological and epithelial barrier disorders, and CamelBio can further supply validated antibody pairs, bulk monoclonal or polyclonal antibodies, and specialized raw materials for test development. Whether you need rare-target sourcing or scaled-up production, our platform bridges the gap between innovation and reliable supply.