Anti-INTS8 Rabbit Polyclonal Antibody for WB, ELISA - Q75QN2

Core Product Specifications and Parameters

Biological Background: INTS8 Function and Localization

• INTS8 (Integrator complex subunit 8), also known as protein kaonashi-1, is a core component of the integrator complex, a multi-subunit assembly that terminates RNA polymerase II (Pol II) transcription at promoter-proximal regions. Related references: PMID:28542170 PMID:33243860 PMID:34004147
• INTS8 is specifically required for the recruitment of protein phosphatase 2A (PP2A) to the transcription pause-release checkpoint, a critical step in quality control during elongation. Related references: PMID:32966759 PMID:33243860 PMID:34004147
• The integrator complex terminates transcription via three coordinated enzymatic activities: CTD dephosphorylation of Pol II and SUPT5H, endonucleolytic cleavage of nascent RNA, and DNA release of the polymerase. Related references: PMID:33243860 PMID:34004147 PMID:38570683
• Beyond mRNA synthesis, INTS8-containing integrator also terminates transcription of non-coding Pol II products, including enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs, and long non-coding RNAs (lncRNAs). Related references: PMID:16239144
• INTS8 localizes to the nucleus and is found on chromosomes, consistent with its direct involvement in transcription regulation.
• The protein contains tetratricopeptide (TPR) repeat motifs, which are likely to mediate protein-protein interactions essential for integrator complex assembly.
• Alternative splicing generates multiple INTS8 isoforms, and the protein is subject to phosphorylation; pathogenic variants in INTS8 are associated with intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 600-800 of human INTS8, a region containing TPR repeats that may be accessible under denaturing conditions, supporting use in Western blotting (WB).
• For WB, consider using whole-cell lysates from human or mouse cell lines; validate antibody specificity with recombinant INTS8 or knockdown/knockout controls in your experimental system.
• This polyclonal antibody can be employed for ELISA-based detection of INTS8; pairing with a matched detection antibody may improve sensitivity and dynamic range.
• Optimize antibody dilution and incubation conditions for your specific sample type and application, as performance can vary with target abundance and matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, technical services, and consulting, supporting every stage from concept to clinic. This anti-INTS8 antibody extends our portfolio for investigating transcription termination and RNA processing pathways. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare target raw materials to accelerate your assay development and commercialization.