Anti-INTS6 Rabbit Polyclonal Antibody for WB, ELISA - Q9UL03

Core Product Specifications and Parameters

Biological Background: INTS6 Function and Localization

• INTS6 is a core component of the integrator complex, which governs RNA polymerase II (Pol II) transcription termination at promoter-proximal regions, acting as a quality checkpoint during elongation (PubMed:33243860, PubMed:34004147, PubMed:39504960). Related references: PMID:33243860, PMID:34004147, PMID:39504960
• The integrator complex terminates transcription through three coordinated activities: dephosphorylation of the Pol II C-terminal domain and SPT5, endonucleolytic cleavage of the nascent RNA, and release of Pol II from DNA (PubMed:33243860, PubMed:34004147, PubMed:38570683). Related references: PMID:33243860, PMID:34004147, PMID:38570683
• INTS6 specifically functions as a molecular adapter, promoting the assembly of protein phosphatase 2A (PP2A) subunits onto the integrator core complex, thereby facilitating PP2A recruitment to transcription pause-release sites (PubMed:33243860, PubMed:34004147). Related references: PMID:33243860, PMID:34004147
• The integrator complex also terminates synthesis of various non-coding Pol II transcripts, including enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs, and long non-coding RNAs (lncRNAs) (PubMed:16239144). Related references: PMID:16239144
• Additionally, INTS6 may mediate recruitment of cytoplasmic dynein to the nuclear envelope, likely through the integrator complex, impacting nuclear positioning (PubMed:23904267). Related references: PMID:23904267
• Widely expressed across tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas, with strong nuclear and chromosomal localization.
• Exhibits a potential tumor suppressor role, as ectopic expression has been shown to suppress tumor cell growth (PubMed:15254679, PubMed:16239144). Related references: PMID:15254679, PMID:16239144
• Post-translational modifications include phosphorylation, and multiple isoforms exist due to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 700-887 of human INTS6 (C-terminal region). This design likely provides specificity for detecting full-length INTS6 in human, mouse, and rat samples.
• For Western blotting, a band around 100 kDa is expected. Optimize sample preparation and gel percentage to resolve high-molecular-weight proteins.
• Validate antibody performance in your specific experimental system (e.g., cell lines, tissue lysates) and consider appropriate loading controls.
• For ELISA applications, titrate the antibody to determine the optimal working concentration and ensure compatibility with your detection reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including the INTS6 rabbit polyclonal antibody. Our portfolio supports integrator complex and transcription termination studies with validated antibodies, bulk ancillary reagents, and rare target sourcing. Partner with us to accelerate your development from concept to clinic with reliable, high-quality materials.