Anti-INTS6 Polyclonal Antibody for WB, ELISA - Q9UL03

Core Product Specifications and Parameters

Biological Background: INTS6 Function and Localization

• INTS6 is a component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:33243860, PubMed:34004147, PubMed:39504960). Related references: PMID:33243860 PMID:34004147 PMID:39504960
• The integrator complex provides a quality checkpoint during transcription elongation by driving premature termination of unfavorably configured transcripts, via dephosphorylation of Pol II, nascent RNA degradation, and release of Pol II from DNA (PubMed:33243860, PubMed:34004147, PubMed:38570683). Related references: PMID:33243860 PMID:34004147 PMID:38570683
• INTS6 acts as a molecular adapter that promotes the assembly of protein phosphatase 2A (PP2A) subunits to the integrator core complex, facilitating PP2A recruitment to the transcription pause-release checkpoint (PubMed:33243860, PubMed:34004147). Related references: PMID:33243860 PMID:34004147
• INTS6 mediates the recruitment of cytoplasmic dynein to the nuclear envelope, likely as part of the integrator complex (PubMed:23904267). Related references: PMID:23904267
• INTS6 may function as a tumor suppressor; ectopic expression suppresses tumor cell growth (PubMed:15254679, PubMed:16239144). Related references: PMID:15254679 PMID:16239144
• INTS6 is localized in the nucleus and associates with chromosomes.
• It is widely expressed, with expression detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• INTS6 undergoes alternative splicing and is a phosphoprotein; its molecular weight is approximately 100 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–280) of human INTS6. For specific detection, consider validating antibody specificity with full-length protein or known expression systems.
• For Western blotting, a band around 100 kDa is expected. Validate detection in relevant sample systems (e.g., human or mouse cell lines expressing INTS6).
• The antibody is also applicable to ELISA; optimize coating and detection conditions based on the recombinant immunogen or native protein in your assay setting.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, providing diagnostic manufacturers and researchers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. For INTS6-related projects—whether you are studying transcription regulation, tumor suppression, or RNA processing—CamelBio supports your assay development from concept to clinic with reliable supply of critical reagents.