Anti-INTS5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q6P9B9

Core Product Specifications and Parameters

Biological Background: INTS5 Function and Localization

• INTS5 (Integrator complex subunit 5), also known as INT5 or KIAA1698, is a component of the integrator complex, a multiprotein assembly that regulates RNA polymerase II (Pol II) transcription termination.
• The integrator complex terminates Pol II transcription in the promoter-proximal region by catalyzing dephosphorylation of the Pol II C-terminal domain and SUPT5H, endonucleolytic cleavage of nascent RNA, and promoting Pol II release from DNA. Related references: PMID:33243860 PMID:38570683
• INTS5-containing integrator also terminates synthesis of non-coding Pol II transcripts, including enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs, and long non-coding RNAs (lncRNAs). Related references: PMID:16239144
• The protein mediates recruitment of cytoplasmic dynein to the nuclear envelope, likely as part of the integrator complex. Related references: PMID:23904267
• Subcellularly, INTS5 localizes to the nucleus, cytoplasm, and nuclear membrane.
• INTS5 undergoes post-translational modifications, including acetylation and phosphorylation, and contains a transmembrane helix region (UniProt keywords: 3D-structure, Acetylation, Cytoplasm, Membrane, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Transmembrane, Transmembrane helix).

Experimental Guidance and Technical Tips

• For western blotting, an ~108 kDa band corresponding to INTS5 can be expected in human and mouse cell lysates; select appropriate gel percentage and transfer conditions.
• In immunohistochemistry on paraffin-embedded sections, antigen retrieval may be required; always validate staining in a relevant sample system with appropriate controls.
• This polyclonal antibody may be suitable for ELISA-based assays; perform proper titration and include negative and positive controls for your specific experimental setup.
• Given the immunogen design (amino acids 120-400 of human INTS5), cross-reactivity with other family members or isoforms is possible; confirm specificity in your model by knockdown or comparative analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a single point of access to IVD raw materials and technical support, from discovery to clinical application. This anti-INTS5 polyclonal antibody supports research into transcription regulation and the integrator complex. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to accelerate your assay development workflow.