Anti-INTS4 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q96HW7

Core Product Specifications and Parameters

Biological Background: Integrator complex subunit 4 Function and Localization

• Integrator complex subunit 4 (INTS4, also known as INT4, MST093) is a key component of the integrator complex, a large multiprotein assembly that regulates RNA polymerase II (Pol II) transcription termination in the promoter-proximal region of genes. Related references: PMID:29471365, PMID:33243860, PMID:33548203
• The integrator complex functions as a quality checkpoint during transcription elongation, driving premature termination of transcripts with unfavorable configurations. This is achieved by dephosphorylating the Pol II C-terminal domain (CTD) and SUPT5H/SPT5, endonucleolytically cleaving the nascent RNA, and promoting Pol II release from DNA. Related references: PMID:33243860, PMID:38570683
• Beyond its role in terminating coding transcripts, the integrator complex is essential for the 3’-end processing of various non-coding RNAs, including enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs, and long non-coding RNAs (lncRNAs). Related references: PMID:16239144
• INTS4 serves as a structural scaffold within the complex, facilitating the interaction between INTS9 and INTS11, which are critical for the complex’s catalytic functions. Related references: PMID:29471365, PMID:33548203
• INTS4 also mediates the recruitment of cytoplasmic dynein to the nuclear envelope, a process likely involving the integrator complex, highlighting its potential role in nucleocytoplasmic transport. Related references: PMID:23904267
• The protein is predominantly localized in the nucleus but is also detected in the cytoplasm, consistent with its dynamic roles in transcription regulation and nuclear envelope dynamics.
• Post-translational modifications of INTS4 include acetylation and ubiquitin-like (Ubl) conjugation, and the protein undergoes alternative splicing to generate multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-270) of human INTS4. To confirm target specificity, consider performing blocking experiments or validation with recombinant INTS4 protein.
• For Western blotting, the predicted molecular weight of endogenous INTS4 is ~108 kDa. An appropriate loading control (e.g., GAPDH or β-actin) should be included to ensure equal loading.
• This antibody is validated for WB, IP, and ELISA. In IP experiments, pre-clearing and optimizing the antibody-to-lysate ratio may improve the signal-to-noise ratio. For ELISA, consider using this polyclonal antibody as a capture or detection reagent with careful titration.
• Cross-reactivity with mouse and rat orthologs is reported, but validate the antibody in your specific model system and application before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, research labs, and institutes from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials. For transcription regulation and RNA processing research, we offer reliable immune reagents like the INTS4 rabbit polyclonal antibody to accelerate your assay development and biomarker studies.