Anti-INTS11 Rabbit Polyclonal Antibody for WB, ELISA - Q5TA45

Core Product Specifications and Parameters

Biological Background: INTS11 Function and Localization

• INTS11 (Integrator complex subunit 11), also known as CPSF3L/RC-68, is the RNA endonuclease component of the integrator complex, which mediates transcription termination of RNA polymerase II (Pol II) at promoter-proximal regions (PubMed:16239144, PubMed:25201415, PubMed:28396433). Related references: PMID:16239144, PMID:25201415, PMID:28396433
• The integrator complex enforces a quality checkpoint during transcription elongation by dephosphorylating the Pol II CTD, degrading nascent RNA via INTS11's endonuclease activity, and promoting Pol II release from DNA (PubMed:32697989, PubMed:33243860, PubMed:33548203). Related references: PMID:32697989, PMID:33243860, PMID:33548203
• INTS11 processes multiple non-coding RNA classes, including small nuclear RNAs (snRNAs), enhancer RNAs (eRNAs), telomerase RNAs, and long non-coding RNAs (lncRNAs), ensuring proper gene expression regulation (PubMed:16239144, PubMed:22252320, PubMed:26308897, PubMed:28396433). Related references: PMID:16239144, PMID:22252320, PMID:26308897, PMID:28396433
• Beyond transcription termination, INTS11 facilitates recruitment of cytoplasmic dynein to the nuclear envelope, likely as part of the integrator complex, linking RNA processing to cytoskeletal organization (PubMed:23904267). Related references: PMID:23904267
• Subcellularly, INTS11 localizes to both the nucleus and cytoplasm, consistent with its dual roles in nuclear RNA processing and dynein-mediated processes.
• INTS11 is a zinc-dependent hydrolase and is subject to ubiquitin-like conjugation; mutations are associated with intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 331–600) of human INTS11, which is conserved across species; cross-reactivity with mouse and rat is expected based on sequence alignment, but validation in these model systems is recommended.
• For Western blot, a specific band at approximately 68 kDa should be detected; loading controls and lysate preparation should be optimized for the target tissue or cell line.
• In ELISA applications, consider using recombinant INTS11 protein as a positive control and titrate both coating antigen and antibody concentrations for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including polyclonal and monoclonal antibodies for transcription and RNA processing targets like INTS11. Our supply chain covers validated antibody pairs, bulk ancillary reagents, and sourcing for rare targets, streamlining your development from concept to clinic.