Anti-INTS11 Monoclonal Antibody for WB, ELISA - Q5TA45

Core Product Specifications and Parameters

Biological Background: INTS11 Function and Localization

• INTS11 is the catalytic RNA endonuclease subunit of the integrator complex, which terminates RNA polymerase II transcription in the promoter-proximal region of genes. Related references: PMID:16239144 PMID:25201415 PMID:28396433
• It degrades the nascent RNA transcript and promotes Pol II release via dephosphorylation of the CTD domain of POLR2A/RPB1 and SUPT5H/SPT5. Related references: PMID:32697989 PMID:33243860 PMID:33548203
• The integrator complex also processes non-coding Pol II transcripts, including snRNAs, eRNAs, telomerase RNAs, and lncRNAs. Related references: PMID:22252320 PMID:26308897
• INTS11 mediates recruitment of cytoplasmic dynein to the nuclear envelope, likely as part of the integrator complex. Related references: PMID:23904267
• The protein localizes to both the nucleus and cytoplasm.
• INTS11 is a zinc-dependent hydrolase with a metalloprotease-like fold, as indicated by structural studies and metal-binding motifs.
• Alternative splicing generates multiple isoforms of INTS11.
• Post-translational modifications include isopeptide bond formation and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 331–600) covers a central region of INTS11 that includes the catalytic domain; this region is highly conserved among human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, a band around 68 kDa is anticipated; verify specificity using appropriate controls and lysates.
• ELISA can be employed to quantify INTS11 in cell or tissue extracts; consider pairing with a relevant detection antibody and validating in the intended sample system.
• Due to the antibody’s reactivity with multiple species, it may be suitable for cross-species comparative studies; always confirm performance in the specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering this anti-INTS11 antibody as part of a broader portfolio for transcription and RNA processing research. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare-target sourcing to streamline your assay development. Whatever your diagnostic manufacturing or laboratory needs, CamelBio bridges the gap from concept to clinic with reliable supply and expert support.