Anti-INTS10 Rabbit Polyclonal Antibody for WB, ELISA - Q9NVR2

Core Product Specifications and Parameters

Biological Background: INTS10 (Integrator complex subunit 10) Function and Localization

• INTS10 is a core component of the integrator complex, which terminates RNA polymerase II transcription in promoter-proximal regions and provides a quality checkpoint during elongation. Related references: PMID:38570683
• The integrator complex also silences non-coding transcripts such as enhancer RNAs, snRNAs, telomerase RNAs, and lncRNAs. Related references: PMID:16239144, PMID:32647223
• Within the integrator tail module, INTS10 serves as a scaffold for recruiting transcription factors to promoters. Related references: PMID:38823386
• INTS10 may not be required for dynein recruitment to the nuclear envelope, a function linked to the integrator complex. Related references: PMID:23904267
• The protein is localized to the nucleus, consistent with its role in transcription regulation.
• INTS10 undergoes post-translational modifications including phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, as indicated by UniProt keywords.
• The gene INTS10 (also known as C8orf35) encodes a protein with a predicted molecular weight of approximately 82 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 451–710 of human INTS10, covering the C-terminal region. This design targets a distinct domain and may allow detection of both full-length and certain truncated variants.
• For Western blotting, a band of approximately 82 kDa is expected based on the predicted molecular weight. Optimize blocking and antibody dilution using relevant cell or tissue lysates from human, mouse, or rat.
• In ELISA, use purified recombinant INTS10 protein or peptide as coating antigen to establish a standard curve. Ensure proper negative controls.
• As a polyclonal antibody, it may exhibit batch-to-batch variation. Validate specificity in your experimental system, e.g., by siRNA knockdown or comparison with knockout models.
• Since INTS10 functions in a large multiprotein complex, this antibody may be suitable for immunoprecipitation of the integrator complex, though this application has not been explicitly validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete suite of IVD raw materials, including this anti-INTS10 polyclonal antibody for transcription and gene regulation research. Our procurement network supplies validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents to accelerate your diagnostic development. Rely on CamelBio as your single contact for sourcing rare targets and scaling up from concept to clinic.