Anti-Interferon alpha-2 (IFN-α2) Monoclonal Antibody for WB, IHC-P, ELISA - P01563

Core Product Specifications and Parameters

Biological Background: Interferon alpha-2 Function and Localization

• Interferon alpha-2 (IFN-α2) is a cytokine belonging to the type I interferon family, encoded by the IFNA2 gene, with isoforms IFNA2A, IFNA2B, and IFNA2C.
• It is primarily produced by macrophages and exhibits potent antiviral activities.
• The protein contains a signal peptide and is secreted into the extracellular space.
• It is a glycoprotein with one disulfide bond, contributing to its structural stability.
• IFN-α2 plays a critical role in innate immune defense against viral infections and is used as a pharmaceutical agent.
• The mature protein has a molecular weight of approximately 21.6 kDa (UniProt), though observed at around 22 kDa under reducing conditions.

Experimental Guidance and Technical Tips

• The immunogen comprises the mature IFN-α2 sequence (amino acids 24-188), which excludes the signal peptide, ensuring reactivity with the secreted protein.
• For Western blotting, a band corresponding to approximately 22 kDa is expected; consider using reducing conditions to optimize detection.
• For immunohistochemistry, antigen retrieval methods such as heat-induced epitope retrieval may enhance staining; validate in tissue samples known to express IFN-α, such as immune organs.
• For ELISA, this monoclonal antibody can be explored as a capture or detection reagent when paired with another anti-IFN-α2 antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a trusted partner, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and offers rare target raw-material sourcing to accelerate your immunoassay development projects focused on interferon alpha-2 and related cytokines.