Anti-Interferon alpha 1 (IFN-α1) Rabbit Polyclonal Antibody for WB, ELISA - P01562

Core Product Specifications and Parameters

Biological Background: Interferon Alpha 1 Function and Localization

• Interferon alpha 1 (IFN-α1) is encoded by the IFNA1 gene (Gene ID: 3439) in humans.
• Alternative names for this target include IFL, IFN, IFNA@, IFNA13, leIF D, IFN-ALPHA, and IFN-alphaD.
• The protein has a predicted molecular weight of approximately 22 kDa.
• The SWISS accession P01562 is currently marked as inactive in the UniProt database; therefore, detailed functional annotation, subcellular localization, and tissue specificity are not available from this source.
• Given its classification as a type I interferon, IFN-α1 is generally implicated in antiviral responses and immune modulation, but specific functional data could not be retrieved for this entry.
• Researchers are encouraged to consult alternative databases or the primary literature for functional insights.
• The immunogen region (amino acids 24-189) encompasses the mature peptide, suggesting the antibody may detect the processed, secreted form of the protein.
• No additional keywords or post‑translational modification data were provided in the source record.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen covering the mature chain (aa 24–189) indicates the antibody likely targets the secreted form; consider using recombinant mature IFN-α1 protein as a positive control in experiments.
• For Western blot, validate detection in human cell lines or tissue samples known to express IFNA1 under induced conditions (e.g., viral stimulation) and include a suitable loading control.
• In ELISA, optimize antigen coating concentration and antibody dilution using checkerboard titrations; note that polyclonal antibodies may require cross-adsorption to reduce background from nonspecific binding.
• The predicted molecular weight is 22 kDa; however, post‑translational modifications such as glycosylation may cause the band to shift. Verify the observed molecular weight against known standards.
• If no signal is observed, consider that IFN-α1 expression may be low or transient; pretreatment with interferon inducers (e.g., poly I:C) may be necessary for detection.
• Ensure samples are not subjected to repeated freeze–thaw cycles, as interferons are susceptible to degradation.
• No cross‑reactivity data aside from human are provided; when working with other species, validate specificity empirically in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized rare-target sourcing. The anti-IFN-α1 rabbit polyclonal antibody supports cytokine research and immunoassay development. Contact us to explore how we can support your diagnostic kit development from concept to clinic.