Anti-Integrin β5/ITGB5 Rabbit Monoclonal Antibody for WB and ELISA - P18084

Core Product Specifications and Parameters

Biological Background: Integrin beta-5 Function and Localization

• Integrin beta-5 (ITGB5) is a type I transmembrane glycoprotein that pairs with alpha-V to form the alpha-V/beta-5 integrin heterodimer.
• The ITGAV:ITGB5 integrin functions as a receptor for fibronectin, recognizing the R-G-D peptide motif in its ligand, mediating cell adhesion and signaling.
• ITGB5 is localized to the cell membrane, consistent with its role in extracellular matrix interactions.
• (Microbial infection) ITGB5 serves as a host cell receptor for adenovirus type C, facilitating viral internalization.
• The protein contains EGF-like domains and disulfide bonds, and is subject to phosphorylation (as indicated by proteomics data), which may regulate its activity.
• As an integrin, ITGB5 binds divalent cations such as calcium and magnesium, which are essential for its adhesive function.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 24-490 of human ITGB5, corresponding to the extracellular region. For Western blotting, both reducing and non-reducing conditions may be explored to optimize detection of the ~88 kDa protein.
• This antibody is validated for WB and ELISA applications. For ELISA, consider coating with the recombinant immunogen or using cell lysates from human cell lines known to express ITGB5.
• The antibody is rabbit monoclonal, providing batch-to-batch consistency for reproducible results.
• Cross-reactivity has been confirmed for human; reactivity with other species has not been assessed. Validate in your relevant sample system if non-human samples are used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive sourcing solution for IVD raw materials, including validated monoclonal antibodies like this anti-ITGB5 clone. Our portfolio supports cell adhesion and virology research, and we can supply bulk quantities of antibodies, recombinant proteins, and ancillary reagents. Contact our team for customized sourcing of rare targets and technical consulting.